SLU-PP-332 (5mg Vial) Dosage Protocol
Experimental ERR-agonist "exercise mimetic" (small molecule) — preclinical (mouse) only; not approved.
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers. Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →Mix & measure SLU-PP-332 · 5 mg
Dosing & Reconstitution Guide
A single practical dilution with accurate once-daily dosing, step by step
Murine-Equivalent Schedule (3 mL = ~1.67 mg/mL)
Reconstitute: Add 3.0 mL bacteriostatic water to one 5 mg vial → final concentration ~1.67 mg/mL (1,667 mcg/mL).
Murine daily dose: 1,250–2,500 mcg/day (murine-equivalent for a ~25 g mouse), given as two divided doses. This is not a human dose.
Easy measuring: At ~1.67 mg/mL, 1 unit ≈ 16.67 mcg on a U-100 syringe (units = mcg ÷ 16.67). The large 3.0 mL dilution keeps every dose at 37.5 units or more for accurate measuring.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the mixed solution.
Why SLU-PP-332 draws research interest
These are the directions researchers and the peptide community most often explore SLU-PP-332 for — so you know you’re in the right place. They describe what is being studied, not proven benefits, approved uses, or promised results.
Fat & weight loss
Studied in preclinical (mouse) research as an ERR-agonist "exercise mimetic" for effects on body fat and weight; animal-only, not approved.
Endurance & exercise capacity
Investigated in animal studies for running endurance and exercise-mimetic effects on physical performance; preclinical and not approved.
Mitochondrial & fat metabolism
Explored in mouse research on mitochondrial activity and fat oxidation through ERR pathway activation; investigational, animal-only.
Cardiac / heart failure
Researched in preclinical rodent models for effects on cardiac function and heart failure; mouse-only and not approved for any use.
Evidence ranges from early laboratory work to clinical trials depending on the use — the sections below cover the actual data and sources.
Quickstart Highlights
SLU-PP-332 is a synthetic small molecule (not a peptide) that acts as a pan-agonist of the estrogen-related receptors ERRα/β/γ — an “exercise mimetic” developed in the Burris/Elgendy lab at Saint Louis University[1][5]. All evidence is preclinical — in mice only. The schedule below reproduces published murine-equivalent regimens (mg/kg-derived for a ~25 g mouse) and is not a recommended human protocol. It is an unapproved research chemical, not a medicine, with no human trials and no FDA approval — presented for research and educational use only.
Add 3.0 mL bacteriostatic water to one 5 mg vial → ~1.67 mg/mL (1,667 mcg/mL), the largest practical dilution for accurate dosing.
1,250–2,500 mcg/day (murine-equivalent, for a ~25 g mouse), split into two divided doses — not a human dose.
At ~1.67 mg/mL, 1 unit ≈ 16.67 mcg; 625 mcg ≈ 37.5 units and 1,250 mcg ≈ 75 units on a U-100 syringe.
Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the solution.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
Route & frequency: published murine studies used intraperitoneal administration twice daily (25–50 mg/kg)[1][2]. The daily mcg values above are murine-equivalent (expressed for a ~25 g mouse), not approved human doses. No reputable source supports a human protocol for this compound.
Reconstitution Steps
Draw 3.0 mL of bacteriostatic water into a sterile syringe.
Release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.
The 3.0 mL dilution is deliberately large so each dose reads at 37.5 units or more, where U-100 syringe markings are most precise. Avoid freezing the reconstituted solution and protect it from light. SLU-PP-332 is a small molecule, not a peptide.
Supplies Needed
Quantities below assume an 8–16 week murine-equivalent course with twice-daily administration.
Based on the murine-equivalent maintenance dose of 2,500 mcg/day from one 5 mg vial.
- 8 weeks: ~25 vials
- 12 weeks: ~39 vials
- 16 weeks: ~53 vials
- Per day: 2 syringes (twice daily)
- 8 weeks: ~112 syringes
- 16 weeks: ~224 syringes
Use ~3.0 mL per 5 mg vial for reconstitution.
- 8 weeks (25 vials): ~75 mL → 8 bottles
- 16 weeks (53 vials): ~159 mL → 16 bottles
One for the vial stopper + one for the injection site each administration.
- Per administration: 2 swabs (4/day)
- 8 weeks: ~224 swabs → 3 boxes
Protocol Overview
A concise summary of the murine-based regimen reproduced from published preclinical studies.
- ▪Mechanism: Pan-agonist of estrogen-related receptors (ERRα/β/γ) that activates an aerobic-exercise gene program and boosts mitochondrial function — reported in mice only, not established in humans[1][2].
- ▪Published route: Intraperitoneal administration in mice, twice daily (25–50 mg/kg); cycle length was 8 weeks in published studies.
- ▪Murine dose range: 1,250–2,500 mcg/day (for a ~25 g mouse) in two divided doses — murine-equivalent, not a human dose.
- ▪Reconstitution: 3.0 mL bacteriostatic water per 5 mg vial gives ~1.67 mg/mL for accurate unit measurements.
- ▪Storage: Keep the dry vial frozen at −20 °C (−4 °F); once mixed, refrigerate at 2–8 °C and do not freeze the solution.
Dosing Protocol
The murine-equivalent dosing framework reproduced from published preclinical studies (not a human protocol).
- ▪Initial phase (Weeks 1–2): 1,250 mcg/day — 625 mcg per injection, twice daily.
- ▪Maintenance phase (Weeks 3–8): 2,500 mcg/day — 1,250 mcg per injection, twice daily.
- ▪Route: Intraperitoneal in published murine models; the doses above are murine-equivalent (for a ~25 g mouse), not human doses.
- ▪Cycle length: 8 weeks in published studies; some research extends to 12–16 weeks.
- ▪Frequency & timing: Twice per day at consistent intervals, reflecting published murine regimens.
Storage Instructions
Correct storage is what preserves the compound’s stability and activity.
- ▪Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions and limit moisture exposure[7].
- ▪Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; avoid freeze–thaw cycles of the mixed solution[8].
- ▪Handling: Let frozen vials warm to room temperature before opening so condensation won’t form, and keep the solution clear of heat and direct light.
- ▪Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.
Important Notes
Practical points that keep daily administration safe and consistent.
- ▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
- ▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[9].
- ▪Slow injection: Push the plunger slowly and pause a few seconds before withdrawing the needle to prevent backflow.
- ▪Recordkeeping: Log the daily dose, injection site and any observations to keep the protocol consistent.
- ▪Regulatory note: SLU-PP-332 is a WADA-prohibited substance (S4.5 metabolic modulators) and is not FDA-approved; there are no human trials and no established human dose[10].
How This Works
SLU-PP-332 is a synthetic small molecule (not a peptide) that acts as a pan-agonist of the estrogen-related receptors ERRα, ERRβ and ERRγ, with strongest activity at ERRα[1][2]. It was developed in the Burris/Elgendy lab at Saint Louis University.
As an “exercise mimetic,” it engages metabolic pathways normally activated by physical training. In mouse studies, acute administration switched on an aerobic-exercise transcriptional program, increased mitochondrial respiration and fatty-acid oxidation in skeletal muscle, and improved endurance and cardiometabolic measures — without the mechanical stress of actual exercise[3][6].
Additional mouse work has reported reversal of mitochondrial dysfunction and inflammation in aging kidney models, alleviation of metabolic syndrome, and improved cardiac fatty-acid metabolism in heart-failure models[4]. Mouse studies used intraperitoneal injection; some users take it orally, but no oral protocol is established.
Critical caveat: all evidence is preclinical, in mice only. There are no human trials, no FDA approval, and no established human dose for SLU-PP-332. The murine benefits should be read as hypotheses; there is no basis to assume they translate to humans.
SLU-PP-332 is not an approved medicine. It is an unapproved research chemical presented here for research and educational purposes only — not for human consumption.
Lifestyle Factors
Contextual factors relevant to preclinical research with this compound.
- ▪Exercise context: SLU-PP-332 was studied alongside exercise training in some murine models, suggesting possible synergistic metabolic effects.
- ▪Diet: Diet composition (standard chow vs. high-fat diet) influenced metabolic outcomes in published mouse studies.
- ▪Environment: Standardize temperature and housing conditions, which can affect metabolic readouts in research protocols.
- ▪Handling: Compound stability and consistent handling procedures are critical for reproducible results.
Potential Benefits & Side Effects
What murine studies describe. There is no human evidence; do not assume these effects translate to humans.
Potential Benefits
- ▪Metabolic (mice): Enhanced mitochondrial function, increased fatty-acid oxidation and improved glucose tolerance in diet-induced obese mice[2].
- ▪Cardiac & renal (mice): Ameliorated heart failure via cardiac fatty-acid metabolism, and reversed mitochondrial dysfunction in aging kidney models[3][4].
- ▪Endurance (mice): Increased treadmill running capacity and muscle oxidative capacity in mice[1].
- ▪Human safety unknown: No human data on tolerability, pharmacokinetics or adverse effects exist; no clinical trials have been conducted[13].
Common Side Effects
- ▪Injection-site reactions: Mild redness, tenderness or soreness can occur; rotating sites helps.
- ▪Unknown long-term profile: Human safety data is limited, so caution and monitoring are advised.
- ▪Sport restriction: SLU-PP-332 is a WADA-prohibited substance (S4.5 metabolic modulators) for athletes.
Injection Technique
General subcutaneous technique, following established clinical best-practice guidance[14][15]. Note: published murine studies used intraperitoneal administration; the subcutaneous route is not validated for this compound.
Pre-Injection Preparation
- ▪Wash your hands well with soap and water.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[15].
- ▪Draw the intended dose, then check for air bubbles and push any out.
Injection Procedure
- ▪Pinch a skinfold at the chosen site between thumb and forefinger.
- ▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[14].
- ▪Skip aspiration for subcutaneous shots — it isn’t needed[14].
- ▪Press the plunger slowly and steadily until it’s fully down.
- ▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
Post-Injection Care
- ▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away.
- ▪Rotate the injection site each day to prevent irritation and lipohypertrophy[9].
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
For high-purity research peptides, we point researchers to Prime Lab Peptides for SLU-PP-332 (5 mg).
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
ACS Chemical Biology (2023)Synthetic ERRα/β/γ agonist induces an ERRα-dependent acute aerobic-exercise response and enhances exercise capacity (SLU-PP-332).
- 2
J. Pharmacology and Experimental Therapeutics (2024)A synthetic ERR agonist (SLU-PP-332) alleviates metabolic syndrome in mice.
- 3
Circulation (2024)Novel pan-ERR agonists ameliorate heart failure through enhanced cardiac fatty-acid metabolism and mitochondrial function (mice).
- 4
American Journal of Pathology (2023)Estrogen-related receptor agonism reverses mitochondrial dysfunction and inflammation in the aging kidney (mice).
- 5
Cell Metabolism / Burris Lab reviewOverview of SLU-PP-332 as a small-molecule pan-ERR agonist and “exercise mimetic” (preclinical).
- 6
Nature Metabolism / endurance studiesSLU-PP-332 increases mitochondrial biogenesis, fatty-acid oxidation and endurance capacity in mice.
- 7
Peptide Storage GuideBest practices for storing lyophilized peptides (temperature, humidity and light protection).
- 8
Bacteriostatic Water GuidanceBacteriostatic water for injection: multi-dose vial stability and handling.
- 9
NCBI BookshelfBest practices for subcutaneous injection: aseptic technique and site rotation.
- 10
WADA Prohibited ListClassification of SLU-PP-332 as a prohibited substance (S4.5 metabolic modulators) in competitive sport.
- 11
Burris/Elgendy Lab, Saint Louis UniversityDiscovery and characterization of SLU-PP-332 as a pan-ERR agonist (synthetic small molecule).
- 12
Acta Pharmacologica Sinica (2015)The multiple universes of estrogen-related receptor α and γ in metabolic control and related diseases.
- 13
ClinicalTrials.govClinical-trial registry: no registered or completed human trials of SLU-PP-332 as of 2025.
- 14
Centers for Disease Control and Prevention (CDC)Subcutaneous injection technique: angle, site and no-aspiration guidance.
- 15
Subcutaneous Injection Technique (Patient Education)How to administer a subcutaneous injection: clinical technique guidelines.
- 16
Prime Lab PeptidesSLU-PP-332 (5 mg) supplier — purity specifications and certificates of analysis.
SLU-PP-332 — frequently asked questions
How do I reconstitute a 5 mg vial of SLU-PP-332?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to SLU-PP-332?
There is no single correct amount — more water simply spreads the same 5 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store SLU-PP-332 after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 5 mg vial of SLU-PP-332 provide?
Divide the vial strength of 5 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is SLU-PP-332 approved for human use?
No. SLU-PP-332 is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
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