5-Amino-1MQ (50mg Vial) Dosage Protocol
Experimental NNMT-inhibitor small molecule studied for fat metabolism — preclinical only, not approved.
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers. Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →Mix & measure 5-Amino-1MQ · 50 mg
5-Amino-1MQ Dosage Chart & Reconstitution Guide
A single practical dilution with simple unit math, step by step
Subcutaneous Approach (4 mL = 12.5 mg/mL)
Reconstitute: Add 4.0 mL bacteriostatic water to one 50 mg vial → final concentration 12.5 mg/mL.
Typical daily range: 2.5–5 mg per day — 2.5 mg/day to start, increasing to 5 mg/day, or split as 2.5 mg twice daily.
Easy measuring: At 12.5 mg/mL, 1 unit = 0.01 mL = 0.125 mg on a U-100 syringe. Units = mg ÷ 0.125.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F), protect from light, and use within 2–4 weeks.
Why 5-Amino-1MQ draws research interest
These are the directions researchers and the peptide community most often explore 5-Amino-1MQ for — so you know you’re in the right place. They describe what is being studied, not proven benefits, approved uses, or promised results.
Fat metabolism & fat storage
Investigated in preclinical research as an NNMT inhibitor for how it affects fat cell metabolism and lipid storage.
Body weight & adiposity
Studied in animal models of diet-induced obesity for changes in fat mass and weight; investigational only, not approved.
NAD+ & cellular energy
Explored in research on the NNMT pathway for its influence on NAD+ salvage and cellular energy metabolism, in preclinical work.
Muscle stem cells & regeneration
Researched in animal studies as an NNMT-inhibiting small molecule for effects on muscle stem cell activity and tissue regeneration.
Evidence ranges from early laboratory work to clinical trials depending on the use — the sections below cover the actual data and sources.
Quickstart Highlights
5-Amino-1MQ (5-amino-1-methylquinolinium) is a small molecule — not a peptide — that selectively inhibits nicotinamide N-methyltransferase (NNMT), an enzyme overexpressed in fat tissue. In animal and cell studies, blocking NNMT is theorized to raise intracellular NAD+ and activate SIRT1, which researchers have linked to fat oxidation and preserved lean mass[1][2]. It is not FDA-approved for anything and has no completed human clinical trials — every effect below is preclinical (rodent/cell) only. Note also that 5-Amino-1MQ is well documented as orally bioavailable and is usually sold and dosed as an oral capsule; the subcutaneous approach on this page is this site’s framing, not the standard route. Presented for research and educational use only.
Add 4.0 mL bacteriostatic water to one 50 mg vial → 12.5 mg/mL, a clean concentration for accurate dosing.
2.5–5 mg per day (subcutaneous in this framing), once daily or split as 2.5 mg twice daily.
At 12.5 mg/mL, 1 unit = 0.125 mg; 2.5 mg = 20 units and 5 mg = 40 units on a U-100 syringe.
Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and protect from light.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
Frequency: one or two subcutaneous injections per day in this framing. Because the compound’s reported plasma half-life is roughly 3.8–6.9 hours[4], twice-daily dosing may give more sustained NNMT inhibition. One 50 mg vial provides roughly 10–20 days of material at these research doses. These figures come from preclinical and pharmacokinetic literature, not from approved human dosing — and note the standard route for this molecule is oral.
Reconstitution Steps
Remove the vial from freezer storage (−20 °C / −4 °F) and let it equilibrate at room temperature for 15–20 minutes.
Draw 4.0 mL of bacteriostatic water into a sterile syringe and release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — the solution should be clear. Don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light; use within 2–4 weeks.
The 4.0 mL fill gives a clean 12.5 mg/mL so each milligram maps to whole insulin units (1 unit = 0.125 mg). Do not freeze the reconstituted solution.
Supplies Needed
Quantities below assume research use with the 50 mg vial format at about 5 mg/day.
A 50 mg vial covers roughly 10 days at 5 mg/day, so plan about one vial per 1–2 weeks.
- 1 week (5 mg/day): ~1 vial
- 2 weeks (5 mg/day): ~1 vial
- 4 weeks (5 mg/day): ~2 vials
- Per injection: 1 syringe
- 1 week (once daily): ~7 syringes
- 1 week (BID): ~14 syringes
- 4 weeks (once daily): ~28 syringes
Use ~4.0 mL per 50 mg vial for reconstitution.
- 1–2 weeks (1 vial): ~4 mL → 1 bottle
- 4 weeks (2 vials): ~8 mL → 1 bottle
One for the vial stopper + one for the injection site each administration.
- Per injection: 2 swabs
- 4 weeks (once daily): ~56 swabs → 1 box
Protocol Overview
A concise summary of the once- or twice-daily regimen, drawn from preclinical reference material.
- ▪Goal: Inhibit NNMT to raise intracellular NAD+ and activate SIRT1, theorized to support fat metabolism and preserve lean mass — effects reported in animal and cell studies, not established in humans[1][5].
- ▪Schedule: Once-daily, or split twice daily, in this subcutaneous framing — though the molecule’s standard route is oral.
- ▪Dose Range: 2.5 mg/day to start, increasing toward 5 mg/day; alternatively 2.5 mg twice daily.
- ▪Reconstitution: 4.0 mL bacteriostatic water per 50 mg vial gives 12.5 mg/mL (1 unit = 0.125 mg).
- ▪Storage: Keep the dry vial frozen at −20 °C (−4 °F); once mixed, refrigerate at 2–8 °C, protect from light, and do not freeze the solution.
Dosing Protocol
A suggested daily approach based on preclinical reference doses for this NNMT inhibitor.
- ▪Start: Begin at 2.5 mg once daily for the first 1–2 days to gauge tolerability.
- ▪Standard: Move to 5 mg once daily from day 3 onward.
- ▪Split option: Given the ~3.8–6.9 h half-life, 2.5 mg twice daily may sustain NNMT inhibition more evenly.
- ▪Cycle length: Research schedules commonly run multi-week cycles; a single 50 mg vial supplies ~10–20 days.
- ▪Timing: Administer at consistent times each day and rotate injection sites systematically.
Storage Instructions
Correct storage preserves the compound’s stability and activity.
- ▪Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions and limit moisture exposure[9].
- ▪Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F), protect from light, and use within about 2–4 weeks; do not freeze the mixed solution.
- ▪Handling: Let frozen vials warm to room temperature before opening so condensation won’t form, and keep the solution clear of heat and direct light.
- ▪Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.
Important Notes
Practical points that keep administration safe and consistent.
- ▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
- ▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[12].
- ▪Route note: 5-Amino-1MQ is well documented as orally bioavailable and is typically sold and dosed as an oral capsule. The subcutaneous approach here is this site’s framing, not the established route[4].
- ▪Recordkeeping: Log the daily dose, injection site and any observations to keep the protocol consistent.
- ▪Regulatory note: 5-Amino-1MQ is not FDA-approved for any use and has not been studied in completed human clinical trials; it is an investigational research chemical.
How This Works
5-Amino-1MQ (5-amino-1-methylquinolinium) is a synthetic small molecule — not a peptide — that selectively inhibits nicotinamide N-methyltransferase (NNMT)[1]. NNMT methylates nicotinamide (a form of vitamin B3) using S-adenosylmethionine (SAM) as the methyl donor. In obesity and metabolic dysfunction, NNMT is often overexpressed in adipose tissue, depleting nicotinamide and lowering NAD+ availability[2].
By inhibiting NNMT, 5-Amino-1MQ is theorized to spare nicotinamide for NAD+ synthesis, raising intracellular NAD+ and activating SIRT1 (Sirtuin 1) pathways tied to mitochondrial biogenesis and fat oxidation[3]. In rodent and cell work, NNMT knockdown or inhibition has been described as protecting against diet-induced weight gain and improving metabolic measures without reducing food intake[5][6].
Some preclinical work has also reported preserved or enhanced lean muscle — for example, greater grip strength in aged mice when combined with exercise[7]. These remain animal and cell-culture findings.
Important caveat: there are no completed human clinical trials of 5-Amino-1MQ. Every fat-metabolism, NAD+, SIRT1 and lean-mass effect described above is preclinical (rodent or cell) only and should be read as a hypothesis, not an established human benefit. Nothing here implies weight-loss efficacy in people.
Route: 5-Amino-1MQ is well documented as orally bioavailable and is usually sold and dosed as an oral capsule (commonly ~50–150 mg/day in product use)[4]. This page reframes it as a subcutaneous injection, which is the site’s choice; the standard route for this molecule is oral.
5-Amino-1MQ is not an approved medicine. It is an unapproved investigational research chemical presented here for research and educational purposes only.
Lifestyle Factors
Habits that may support metabolic health alongside the protocol.
- ▪Nutrition: Keep protein intake adequate and overall diet balanced to support metabolism and lean mass.
- ▪Activity: Pair regular resistance and aerobic exercise with the protocol — preclinical lean-mass effects were strongest when combined with exercise.
- ▪Sleep: Aim for 7–9 hours to support the body’s natural metabolic and repair processes.
- ▪Stress: Manage stress with evidence-based practices, since it influences overall metabolic health.
Potential Benefits & Side Effects
What preclinical literature describes. There is no completed human evidence, and individual results are unknown.
Potential Benefits (preclinical)
- ▪Fat metabolism: Animal models report reductions in fat mass while lean muscle is preserved[5][6].
- ▪NAD+ / SIRT1: Associated with elevated NAD+ and SIRT1 activation in preclinical studies[2][3].
- ▪Muscle strength: Enhanced grip strength observed in aged mice when combined with exercise[7].
- ▪Note on humans: These benefits are not established in humans — no completed clinical trials exist[1].
Reported / Possible Side Effects
- ▪Mild, anecdotal: Occasional reports of mild headache, transient jitteriness, or injection-site reactions.
- ▪Injection-site reactions: Redness, tenderness or soreness can occur in this SC framing; rotating sites helps.
- ▪Unknown long-term profile: No human safety data, so caution and monitoring are advised.
Injection Technique
General subcutaneous technique, following established clinical best-practice guidance[10][11]. (The standard route for this molecule is oral; this covers the SC framing used on this page.)
Pre-Injection Preparation
- ▪Wash your hands well with soap and water.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[11].
- ▪Draw the intended dose, then check for air bubbles and push any out.
Injection Procedure
- ▪Pinch a skinfold at the chosen site between thumb and forefinger.
- ▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[10].
- ▪Skip aspiration for subcutaneous shots — it isn’t needed[10].
- ▪Press the plunger slowly and steadily until it’s fully down.
- ▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
Post-Injection Care
- ▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away.
- ▪Rotate the injection site each day to prevent irritation and lipohypertrophy[12].
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
For high-purity research compounds, we point researchers to Prime Lab Peptides.
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality compounds.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
Nature Medicine (2014)Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity (preclinical).
- 2
PMC (2024)NNMT inhibition mitigates obesity-related metabolic dysfunctions in animal models.
- 3
Frontiers in Pharmacology (2024)NNMT as a candidate therapeutic target for metabolic syndrome (review).
- 4
PubMed (2021)LC-MS/MS assay for 5-amino-1-methylquinolinium: pharmacokinetics and oral bioavailability.
- 5
ResearchGate (2021)Combined NNMT inhibition and reduced-calorie diet normalizes body composition in obese mice.
- 6
PMC (2022)Reduced-calorie diet with NNMT inhibition establishes a distinct microbiome in DIO mice.
- 7
NMN.com / Scientific ReportsNNMT inhibition and muscle strength: enhanced grip strength with exercise (preclinical).
- 8
Swolverine5-Amino-1MQ mechanism, benefits, stacking and cycling overview (educational).
- 9
- 10
- 11
CDC (Subcut Injection PDF)Technique diagram and site guidance for subcutaneous injections.
- 12
- 13
Journal of Medicinal Chemistry (ACS)Bisubstrate inhibitors of NNMT with enhanced activity.
- 14
- 15
Prime Lab PeptidesResearch-grade supplier reference for high-purity compounds and batch documentation.
5-Amino-1MQ — frequently asked questions
How do I reconstitute a 50 mg vial of 5-Amino-1MQ?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to 5-Amino-1MQ?
There is no single correct amount — more water simply spreads the same 50 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store 5-Amino-1MQ after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 50 mg vial of 5-Amino-1MQ provide?
Divide the vial strength of 50 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is 5-Amino-1MQ approved for human use?
No. 5-Amino-1MQ is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
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