5-Amino-1MQ (10mg Vial) Dosage Protocol
Experimental NNMT-inhibitor small molecule studied for fat metabolism — preclinical only, not approved.
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers. Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →Mix & measure 5-Amino-1MQ · 10 mg
Dosing & Reconstitution Guide
A single practical dilution with simple unit math, step by step
Standard / Gradual Approach (3 mL = ~3.33 mg/mL)
Reconstitute: Add 3.0 mL bacteriostatic water to one 10 mg vial → final concentration ~3.33 mg/mL.
Typical daily range: 150–600 mcg once daily — 150 mcg/day to start, titrating gradually to 600 mcg/day over 8 weeks.
Easy measuring: At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 mcg on a U-100 syringe. Units = mcg ÷ 33.3.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F), protect from light, and use within 2–4 weeks.
Why 5-Amino-1MQ draws research interest
These are the directions researchers and the peptide community most often explore 5-Amino-1MQ for — so you know you’re in the right place. They describe what is being studied, not proven benefits, approved uses, or promised results.
Fat metabolism & lipolysis
Studied in preclinical adipocyte research as an NNMT inhibitor exploring fat breakdown and cellular energy expenditure.
Weight & obesity
Investigated in animal models of diet-induced obesity; a small molecule that remains preclinical and not approved.
NAD+ & cellular metabolism
Explored in cell research on how NNMT inhibition affects NAD+ and SAM levels — investigational, animal and in-vitro only.
Body composition
Researched in rodent studies on adipocyte size and lean-versus-fat mass; preclinical, not a treatment for any condition.
Evidence ranges from early laboratory work to clinical trials depending on the use — the sections below cover the actual data and sources.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
Frequency: one subcutaneous injection per day in this framing. This stepwise titration lets you monitor tolerance while gradually reaching the effective dosing levels observed in preclinical research[1][4]. One 10 mg vial provides roughly 16–20 days of material at the 600 mcg/day target, and longer during the ramp weeks. For ≤10-unit (≤0.10 mL) administrations, consider 30- or 50-unit insulin syringes for improved readability. These figures come from preclinical and pharmacokinetic literature, not from approved human dosing — and note the standard route for this molecule is oral.
Reconstitution Steps
Remove the vial from freezer storage (−20 °C / −4 °F) and let it equilibrate at room temperature for 15–20 minutes.
Draw 3.0 mL of bacteriostatic water into a sterile syringe and release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — the solution should be clear. Don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light; use within 2–4 weeks.
Do not freeze the reconstituted solution.
Supplies Needed
Quantities below assume research use with the 10 mg vial format across an 8–16 week daily protocol with gradual titration.
A 10 mg vial covers roughly 16–20 days at the 600 mcg/day target, so plan across the titration schedule.
- 8 weeks: ~3 vials
- 12 weeks: ~4 vials
- 16 weeks: ~6 vials
- Per week (once daily): 7 syringes
- 8 weeks: 56 syringes
- 12 weeks: 84 syringes
- 16 weeks: 112 syringes
Use ~3.0 mL per 10 mg vial for reconstitution.
- 8 weeks (3 vials): ~9 mL → 1 bottle
- 12 weeks (4 vials): ~12 mL → 2 bottles
- 16 weeks (6 vials): ~18 mL → 2 bottles
One for the vial stopper + one for the injection site each administration (2/day).
- Per week: 14 swabs
- 8 weeks: 112 swabs → 2 × 100-count boxes
- 12 weeks: 168 swabs → 2 × 100-count boxes
- 16 weeks: 224 swabs → 3 × 100-count boxes
Important Notes
Practical points that keep administration safe and consistent.
- ▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
- ▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[12].
- ▪Route note: 5-Amino-1MQ is well documented as orally bioavailable and is typically sold and dosed as an oral capsule. The subcutaneous approach here is this site’s framing, not the established route[4].
- ▪Recordkeeping: Log the daily dose, injection site and any observations to keep the protocol consistent.
- ▪Regulatory note: 5-Amino-1MQ is not FDA-approved for any use and has not been studied in completed human clinical trials; it is an investigational research chemical.
How This Works
5-Amino-1MQ (5-amino-1-methylquinolinium) is a synthetic small molecule — not a peptide — that selectively inhibits nicotinamide N-methyltransferase (NNMT)[1]. NNMT methylates nicotinamide (a form of vitamin B3) using S-adenosylmethionine (SAM) as the methyl donor. In obesity and metabolic dysfunction, NNMT is often overexpressed in adipose tissue, depleting nicotinamide and lowering NAD+ availability[2].
By inhibiting NNMT, 5-Amino-1MQ is theorized to spare nicotinamide for NAD+ synthesis, raising intracellular NAD+ and activating SIRT1 (Sirtuin 1) pathways tied to mitochondrial biogenesis and fat oxidation[3]. In rodent and cell work, NNMT knockdown or inhibition has been described as protecting against diet-induced weight gain and improving metabolic measures without reducing food intake[5][6].
Some preclinical work has also reported preserved or enhanced lean muscle — for example, greater grip strength in aged mice when combined with exercise[7]. These remain animal and cell-culture findings.
Important caveat: there are no completed human clinical trials of 5-Amino-1MQ. Every fat-metabolism, NAD+, SIRT1 and lean-mass effect described above is preclinical (rodent or cell) only and should be read as a hypothesis, not an established human benefit. Nothing here implies weight-loss efficacy in people.
Route: 5-Amino-1MQ is well documented as orally bioavailable and is usually sold and dosed as an oral capsule (commonly ~50–150 mg/day in product use)[4]. This page reframes it as a subcutaneous injection, which is the site’s choice; the standard route for this molecule is oral.
5-Amino-1MQ is not an approved medicine. It is an unapproved investigational research chemical presented here for research and educational purposes only.
Lifestyle Factors
Habits that may support metabolic health alongside the protocol.
- ▪Nutrition: Keep protein intake adequate and overall diet balanced to support metabolism and lean mass.
- ▪Activity: Pair regular resistance and aerobic exercise with the protocol — preclinical lean-mass effects were strongest when combined with exercise.
- ▪Sleep: Aim for 7–9 hours to support the body’s natural metabolic and repair processes.
- ▪Stress: Manage stress with evidence-based practices, since it influences overall metabolic health.
Potential Benefits & Side Effects
What preclinical literature describes. There is no completed human evidence, and individual results are unknown.
Potential Benefits (preclinical)
- ▪Fat metabolism: Animal models report reductions in fat mass while lean muscle is preserved[5][6].
- ▪NAD+ / SIRT1: Associated with elevated NAD+ and SIRT1 activation in preclinical studies[2][3].
- ▪Muscle strength: Enhanced grip strength observed in aged mice when combined with exercise[7].
- ▪Note on humans: These benefits are not established in humans — no completed clinical trials exist[1].
Reported / Possible Side Effects
- ▪Mild, anecdotal: Occasional reports of mild headache, transient jitteriness, or injection-site reactions.
- ▪Injection-site reactions: Redness, tenderness or soreness can occur in this SC framing; rotating sites helps.
- ▪Unknown long-term profile: No human safety data, so caution and monitoring are advised.
Injection Technique
General subcutaneous technique, following established clinical best-practice guidance[10][11]. (The standard route for this molecule is oral; this covers the SC framing used on this page.)
Pre-Injection Preparation
- ▪Wash your hands well with soap and water.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[11].
- ▪Draw the intended dose, then check for air bubbles and push any out.
Injection Procedure
- ▪Pinch a skinfold at the chosen site between thumb and forefinger.
- ▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[10].
- ▪Skip aspiration for subcutaneous shots — it isn’t needed[10].
- ▪Press the plunger slowly and steadily until it’s fully down.
- ▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
Post-Injection Care
- ▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away.
- ▪Rotate the injection site each day to prevent irritation and lipohypertrophy[12].
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
For high-purity research compounds, we point researchers to Prime Lab Peptides.
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality compounds.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
Nature Medicine (2014)Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity (preclinical).
- 2
PMC (2024)NNMT inhibition mitigates obesity-related metabolic dysfunctions in animal models.
- 3
Frontiers in Pharmacology (2024)NNMT as a candidate therapeutic target for metabolic syndrome (review).
- 4
PubMed (2021)LC-MS/MS assay for 5-amino-1-methylquinolinium: pharmacokinetics and oral bioavailability.
- 5
ResearchGate (2021)Combined NNMT inhibition and reduced-calorie diet normalizes body composition in obese mice.
- 6
PMC (2022)Reduced-calorie diet with NNMT inhibition establishes a distinct microbiome in DIO mice.
- 7
NMN.com / Scientific ReportsNNMT inhibition and muscle strength: enhanced grip strength with exercise (preclinical).
- 8
Swolverine5-Amino-1MQ mechanism, benefits, stacking and cycling overview (educational).
- 9
- 10
- 11
CDC (Subcut Injection PDF)Technique diagram and site guidance for subcutaneous injections.
- 12
- 13
Journal of Medicinal Chemistry (ACS)Bisubstrate inhibitors of NNMT with enhanced activity.
- 14
- 15
Prime Lab PeptidesResearch-grade supplier reference for high-purity compounds and batch documentation.
5-Amino-1MQ — frequently asked questions
How do I reconstitute a 10 mg vial of 5-Amino-1MQ?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to 5-Amino-1MQ?
There is no single correct amount — more water simply spreads the same 10 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store 5-Amino-1MQ after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 10 mg vial of 5-Amino-1MQ provide?
Divide the vial strength of 10 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is 5-Amino-1MQ approved for human use?
No. 5-Amino-1MQ is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
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