TB-500 & BPC-157 Stack Dosage Protocol
Soft-tissue repair stack — TB-500 + BPC-157, two separate research-only vials; the combination is not clinically validated.
Fixed ratio: TB-500 5 mg : BPC-157 5 mg — a 1:1 blend. Every withdrawal keeps that ratio, so the components cannot be dosed independently.
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers. Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →Mix & measure TB-500 + BPC-157 · 5 mg
Dosing & Reconstitution Guide
Reconstitute each peptide in its own vial — never mix the two in one syringe
This stack uses two separate research-only vials — one TB-500 (5 mg) and one BPC-157 (5 mg) — each reconstituted and dosed independently. Two approaches are shown per peptide: a Standard / Gradual reconstitution (2 mL) and an Advanced / Aggressive reconstitution (1 mL). Pair every concentration with its matching recon volume. The combination is not clinically validated and these figures come from reference protocols, not approved human dosing.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
TB-500 (5 mg Vial): Standard / Gradual Approach (2 mL = 2.5 mg/mL)
Reconstitute: Add 2.0 mL bacteriostatic water to the TB-500 (5 mg) vial → 2.5 mg/mL. The full vial holds 5 mg.
Per injection: 2.5 mg twice weekly (5 mg/week total), drawn as ~100 units (1.0 mL) on a U-100 syringe.
Easy measuring: At 2.5 mg/mL, 1 mg ≈ 0.4 mL (40 units); a 2.5 mg dose reads at a full 100 units (1.0 mL).
Storage: Refrigerate the reconstituted vial at 2–8 °C (35.6–46.4 °F), protected from light; do not freeze the solution.
TB-500 (5 mg Vial): Advanced / Aggressive Approach (1 mL = 5 mg/mL)
Reconstitute: Add 1.0 mL bacteriostatic water to the TB-500 (5 mg) vial → 5 mg/mL.
Per injection: 5 mg twice weekly (10 mg/week total), drawn as ~100 units (1.0 mL). If 5 mg in one shot feels too high, split into smaller volumes.
Easy measuring: At 5 mg/mL, 1 mg ≈ 0.2 mL (20 units); a 5 mg dose reads at a full 100 units (1.0 mL).
Storage: Refrigerate at 2–8 °C, protected from light; do not freeze the mixed solution.
BPC-157 (5 mg Vial): Standard / Gradual Approach (2 mL = 2.5 mg/mL)
Reconstitute: Add 2.0 mL bacteriostatic water to the BPC-157 (5 mg) vial → 2.5 mg/mL (2,500 mcg/mL). Ideal for daily or split-daily dosing.
Daily range: 500 mcg/day — either 250 mcg twice daily or 500 mcg once daily.
Easy measuring: At 2,500 mcg/mL, 250 mcg ≈ 10 units (0.10 mL) and 500 mcg ≈ 20 units (0.20 mL) on a U-100 syringe.
Storage: Refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; do not freeze the solution.
BPC-157 (5 mg Vial): Advanced / Aggressive Approach (1 mL = 5 mg/mL)
Reconstitute: Add 1.0 mL bacteriostatic water to the BPC-157 (5 mg) vial → 5 mg/mL (5,000 mcg/mL).
Daily range: Up to 750 mcg/day early (split as ~375 mcg twice daily), tapering to 500 mcg/day.
Easy measuring: At 5,000 mcg/mL, 375 mcg ≈ 7.5 units (0.075 mL) and 500 mcg ≈ 10 units (0.10 mL). Below ~10 units, a 30- or 50-unit syringe improves accuracy.
Storage: Refrigerate at 2–8 °C, protected from light; do not freeze the mixed solution.
Reconstitution Steps (each vial separately)
Draw the chosen volume of bacteriostatic water (1.0 mL or 2.0 mL) for the vial you are reconstituting into a sterile syringe.
Release it slowly down each vial inner wall to limit foaming. Reconstitute TB-500 and BPC-157 in their own separate vials — never combine the powders or solutions in a single vial or syringe.
Swirl or roll gently until fully dissolved — do not shake.
Label each vial with its peptide name, date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.
Keep TB-500 and BPC-157 in separate, clearly labelled vials and draw each into its own syringe. A typical research cycle runs about 4–6 weeks. Avoid freezing reconstituted solution, since freeze–thaw can denature the peptides.
Why TB-500 + BPC-157 draws research interest
These are the directions researchers and the peptide community most often explore TB-500 + BPC-157 for — so you know you’re in the right place. They describe what is being studied, not proven benefits, approved uses, or promised results.
Soft-tissue repair
Investigated in animal studies for tendon, ligament and muscle repair — the pairing most people seek this stack for; not clinically validated.
Injury recovery
Explored in preclinical research on recovery from acute injury and overuse strain; both peptides remain investigational and unapproved.
Anti-inflammatory
BPC-157 in particular is studied in animal research for gut-lining and systemic inflammation; findings are preclinical only, not approved.
Angiogenesis / blood flow
Both peptides are investigated in animal models for angiogenesis, the new-blood-vessel formation thought to support tissue repair.
Evidence ranges from early laboratory work to clinical trials depending on the use — the sections below cover the actual data and sources.
Supplies Needed
Quantities below assume a research cycle of about 4–6 weeks. The stack uses two separate vials — TB-500 (5 mg) injected twice weekly and BPC-157 (5 mg) injected daily.
One TB-500 (5 mg) vial covers about one week at ~5 mg/week; one BPC-157 (5 mg) vial covers ~10 days at 500 mcg/day. Buy them together as a stack.
- 4 weeks: ~4 TB-500 + ~3 BPC-157
- 6 weeks: ~6 TB-500 + ~4 BPC-157
- Buy as a stack: TB-500 + BPC-157 together
- Per injection: 1 syringe (separate vials)
- 4 weeks: ~36 syringes (BPC daily + TB 2×/wk)
- 6 weeks: ~54 syringes
Use 1–2 mL per 5 mg vial (2 mL = standard, 1 mL = advanced). Two vials per cycle stretch.
- ~4 mL per refill (2 mL × 2 vials)
- 4–6 week cycle: ~16–24 mL → 2–3 bottles
One for the vial stopper + one for the injection site each day.
- Per injection: 2 swabs
- 4–6 week cycle: ~100–150 swabs → 1–2 boxes
Storage Instructions
Correct storage preserves the stability and activity of both peptides.
- ▪Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions and limit moisture exposure[7].
- ▪Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F) and use within about 28 days; do not freeze the mixed solution, as freezing can denature peptides[8].
- ▪Handling: Let frozen vials warm to room temperature before opening so condensation won’t form, and keep the solution clear of heat and direct light.
- ▪Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.
Important Notes
Practical points that keep daily administration safe and consistent.
- ▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
- ▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[9].
- ▪Slow injection: Push the plunger slowly and pause a few seconds before withdrawing the needle to prevent backflow.
- ▪Separate vials: Keep TB-500 and BPC-157 in their own clearly labelled vials, draw each into its own syringe, and never mix them in one syringe. Log doses and sites to keep the protocol consistent.
- ▪Regulatory note: Neither peptide is FDA-approved; both are unapproved research chemicals. TB-500 remains a 503A Category-2 bulk substance, while BPC-157 was removed from the 503A list in April 2026 and is under PCAC review. Both are prohibited by WADA (Class S2) for athletic use, and TB-500 is detectable in doping control[10].
How This Works
TB-500 is a synthetic peptide that reproduces the active actin-binding region of Thymosin Beta-4 (Tβ4) — the short sequence Ac-LKKTETQ[1][2]. By sequestering G-actin it helps regulate actin polymerization, the process behind cell migration in wound healing; preclinical work also links it to angiogenesis, stem/progenitor-cell recruitment, and reduced inflammation and fibrosis[5][6].
BPC-157 is a synthetic 15-amino-acid peptide derived from a protective protein found in gastric juice. In animal models it has been associated with tendon, ligament, muscle and gut healing, angiogenesis and cytoprotection — effects reported preclinically, not confirmed in controlled human trials.
The two are stacked on the rationale that TB-500 acts more systemically (cell migration, vascular repair) while BPC-157 is thought to act on localized connective-tissue and gut healing — a proposed “synergistic recovery” combination. This synergy is a hypothesis only.
Important caveat: there is no completed controlled human trial validating the TB-500 + BPC-157 combination, and the limited human data on each peptide individually are minimal. Recovery and healing claims rest on animal data and anecdote and should be read as hypotheses.
Both peptides are not approved medicines. They are unapproved research chemicals presented here for research and educational purposes only.
Lifestyle Factors
Habits that may support recovery alongside the protocol.
- ▪Nutrition: Keep protein intake adequate to give tissue repair the building blocks it needs.
- ▪Activity & rest: Pair appropriate movement with real recovery time and avoid overtraining during an injury-recovery phase.
- ▪Sleep: Aim for 7–9 hours to support the body’s natural repair processes.
- ▪Stress: Manage stress with evidence-based practices, since it influences overall healing.
Potential Benefits & Side Effects
What preclinical literature describes for each peptide; human evidence is limited, the combination is unstudied, and individual results vary.
Potential Benefits
- ▪Soft-tissue repair (preclinical): Animal studies report faster wound healing via TB-500-driven angiogenesis and cell migration, plus BPC-157-associated tendon, ligament and gut healing[5][6].
- ▪Inflammation & vascular support (preclinical): Both peptides have been linked to reduced inflammation and improved angiogenesis in animal models[11].
- ▪Tolerability (preclinical): Each peptide is generally well tolerated in animal studies, with occasional mild injection-site reactions.
- ▪Note on humans: These benefits are not established in humans, and no controlled human trial validates the TB-500 + BPC-157 combination[13].
Common Side Effects
- ▪Injection-site reactions: Mild redness, tenderness or soreness can occur; rotating sites helps.
- ▪Unknown long-term profile: Human safety data is limited, so caution and monitoring are advised.
- ▪Sport restriction: Both TB-500 and BPC-157 are WADA-prohibited (Class S2) for athletes; TB-500 is detectable in testing.
Injection Technique
Because this is a two-vial stack, draw and inject TB-500 and BPC-157 separately — never mix them in one syringe. General subcutaneous technique follows established clinical best-practice guidance[14][15].
Pre-Injection Preparation
- ▪Wash your hands well with soap and water.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[15].
- ▪Draw the intended dose, then check for air bubbles and push any out.
Injection Procedure
- ▪Pinch a skinfold at the chosen site between thumb and forefinger.
- ▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[14].
- ▪Skip aspiration for subcutaneous shots — it isn’t needed[14].
- ▪Press the plunger slowly and steadily until it’s fully down.
- ▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
Post-Injection Care
- ▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away.
- ▪Rotate the injection site each day to prevent irritation and lipohypertrophy[9].
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
For high-purity research peptides, we point researchers to Prime Lab Peptides for the TB-500 (5 mg) + BPC-157 (5 mg) stack — supplied as two separate vials.
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
FASEB JournalBiological activities of thymosin beta-4 mapped to active peptide sequences, including the fragment marketed as TB-500.
- 2
Journal of Chromatography A (PubMed)Doping-control analysis of TB-500 as a synthetic thymosin beta-4 fragment in biological samples.
- 3
WADA Scientific ResearchInvestigation of TB-500 metabolism, synthesis of its metabolites, and detection limits.
- 4
Racing Medication & Testing ConsortiumThymosin beta-4 regulatory bulletin covering TB-500 use in equine sports medicine.
- 5
Journal of Investigative Dermatology (PubMed)Thymosin beta-4 accelerates wound healing in a preclinical model.
- 6
FASEB Journal (PubMed)Active-site mapping of thymosin beta-4 fragments for angiogenesis and cell migration.
- 7
Peptide Storage GuideBest practices for storing lyophilized peptides (temperature, humidity and light protection).
- 8
Bacteriostatic Water GuidanceBacteriostatic water for injection: multi-dose vial stability and handling.
- 9
NCBI BookshelfBest practices for subcutaneous injection: aseptic technique and site rotation.
- 10
WADA Prohibited ListClassification of TB-500 as a prohibited substance in competitive sport.
- 11
Journal of Investigative DermatologyThymosin beta-4 wound-healing mechanisms: collagen deposition, angiogenesis and granulation tissue.
- 12
Journal of Chromatography B (PubMed)Quantification of TB-500 metabolites and screening of wound-healing activity (prodrug hypothesis).
- 13
ClinicalTrials.govTrial registry for full-length thymosin beta-4 (e.g., dermal and ocular indications); no completed efficacy trials of the TB-500 fragment.
- 14
Centers for Disease Control and Prevention (CDC)Subcutaneous injection technique: angle, site and no-aspiration guidance.
- 15
Subcutaneous Injection Technique (Patient Education)How to administer a subcutaneous injection: clinical technique guidelines.
- 17
Journal of Applied Physiology / PubMedBPC-157 preclinical tendon, ligament and muscle healing and angiogenesis in animal models.
- 18
PubMedBPC-157 cytoprotection and gut-healing studies; gastric-derived pentadecapeptide pharmacology.
- 16
Prime Lab PeptidesTB-500 (5 mg) + BPC-157 (5 mg) stack product page — purity specifications and certificates of analysis.
TB-500 + BPC-157 — frequently asked questions
How do I reconstitute a 5 mg vial of TB-500 + BPC-157?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to TB-500 + BPC-157?
There is no single correct amount — more water simply spreads the same 5 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store TB-500 + BPC-157 after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 5 mg vial of TB-500 + BPC-157 provide?
Divide the vial strength of 5 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is TB-500 + BPC-157 approved for human use?
No. TB-500 + BPC-157 is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
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