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Complete guide

Prep & Injection Guide

Everything you need to know about peptide storage, reconstitution, and administration — explained step by step.

06
Sections
02
Injection routes
~18 min
Read time
01
Stability & shelf life

Peptide Storage

Peptides are fragile and require specific, stable storage conditions to retain their potency and maximize shelf life. When you purchase multiple vials, only reconstitute the one you'll be dosing from.

Factors affecting stability
Temperature swings

Higher temperatures accelerate degradation.

Freeze / thaw cycles

One of the fastest ways to reduce potency.

Humidity

Can cause degradation and crystal formation.

Light exposure

Degrades potency over time.

Mechanical shock

Dropping vials can reduce potency.

Storage preparation
1

Get an opaque container

Use one that seals tightly. If it isn't opaque, wrap the peptides in foil.

2

Add desiccant packets

Silica gel packets minimize moisture exposure — worth the small investment.

3

Seal & store properly

Refrigerate or freeze, depending on how long you plan to store it.

Storage duration
State Storage Duration
Reconstituted Fridge36–46°F (2–8°C) 60–90 days
NAD+, AOD-9604 Fridge36–46°F (2–8°C) 30–45 days
Lyophilized Room temp68–77°F (20–25°C) 30–60 days
Lyophilized Fridge36–46°F (2–8°C) Up to 1 year
Lyophilized Freezer-4°F (-20°C) 2–3 years

Lipo-C exception: Do not refrigerate — it will gel. Store at 68–77°F (20–25°C), away from light.

02
What to gather

Supplies Needed

Required

  • Alcohol prep pads
  • Bacteriostatic (BAC) water — or the specific solvent for your peptide
  • Clean insulin syringes — 0.3 mL for single-unit doses

Optional

  • 22–23 gauge, 3 mL syringe for reconstitution
  • Storage container — light-blocking and moisture-proof
03
Tools & measurement

Syringes

Syringe anatomy
Syringe anatomy — tip, barrel and plunger
Tip

The needle end.

Barrel

The cylindrical part marked with unit numbers.

Plunger

The movable part inside the barrel.

Syringe types
1 mL · 100 u
1 mL / 100-unit insulin syringe

1 mL / 100-unit

For doses over 10 units.

Each tick = 2 units Can't measure single units accurately
Best precision
0.3 mL · 30 u
0.3 mL / 30-unit insulin syringe

0.3 mL / 30-unit

For single-unit doses (2, 5, 7 units…).

Each tick = 1 unit Best precision available
3 mL · 22–23 G
3 mL reconstitution syringe

3 mL Reconstitution

For measuring BAC water accurately.

Short lines = 0.1 mL increments
10 mL · Luer
Luer Lock 10 mL syringe

Luer Lock 10 mL

For Lemon Bottle or large volumes.

Needle sold separately

Needle gauge

Higher number = thinner
Needle gauge comparison chart
Reconstitution · 27 G Dosing · 29–31 G

Needle length

Subcutaneous vs intramuscular needle depth
Subcutaneous
5⁄16″ · 8 mm½″ · 12 mm
Intramuscular
½″ · 12 mm recommended
04
Mixing the vial

Reconstitution

1

Remove caps & clean

Clean both vials with alcohol prep pads.

2

Select the needle

A 22–23 G, 3 mL syringe is best. A 28–29 G insulin syringe also works.

3

Draw the water

Insert into the BAC water vial, invert, and draw the indicated amount.

4

Add the water

Insert into the peptide vial. Press the plunger slowly and gently.

5

Remove & dispose

Cap and dispose of the syringe properly.

6

Mix gently

Roll between palms. Let sit 15–30 minutes to fully dissolve.

Tip: Before each dose, roll the vial gently between your palms to ensure even mixing.

05
Under the skin

Subcutaneous Injection

1

Clean vial & draw dose

Use an alcohol pad. Draw the indicated amount from the inverted vial.

2

Prepare the site

Clean the injection site with an alcohol pad.

3

Pinch & inject

Pinch a fold of skin and lift it away from the body. Insert the needle fully with a quick motion.

4

Depress the plunger

Inject the full dose. Wait a few seconds before removing.

5

Post-injection

Rub the site for about a minute. Return the peptide to the fridge.

Best injection sites
Subcutaneous injection sites
Abdomen
Thigh
Back of arm
Lower back
06
Into the muscle

Intramuscular Injection

1

Clean & draw

Clean the vial top. Draw the indicated dose from the inverted vial.

2

Prepare the site

Clean the injection site with an alcohol pad.

3

Inject into muscle

Push the needle quickly through the skin and into the muscle.

4

Complete injection

Depress the plunger fully. Wait a few seconds before removing.

5

Post-injection

Rub the site for about a minute. Return the peptide to the fridge.

Best injection sites
Intramuscular injection sites
Deltoid
Thigh
Gluteal
Methodology & attribution
Compiled by the Dosage Peptide research team from published peptide-reconstitution arithmetic and documented research protocols. Every figure shown is derived, not a clinical recommendation — research use only. Last reviewed July 2026.