NAD+ (500mg Vial) Dosage Protocol
Cellular-energy coenzyme (NAD+) for metabolism/longevity — research/educational dosing reference.
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers. Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →Mix & measure NAD+ · 500 mg
Dosing & Reconstitution Guide
A single practical dilution with accurate once-daily dosing, step by step
Standard / Gradual Approach (5 mL = 100 mg/mL)
Reconstitute: Add 5.0 mL bacteriostatic water to one 500 mg vial → final concentration 100 mg/mL (1 unit = 1 mg).
Typical daily range: 50–100 mg once daily subcutaneously, started low and raised gradually to tolerance over an 8–12 week course.
Easy measuring: At 100 mg/mL, 1 unit = 1 mg on a U-100 syringe, so the dose in milligrams equals the dose in units — verify units = dose ÷ concentration before drawing.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F), protect from light, and do not freeze the mixed solution.
Why NAD+ draws research interest
These are the directions researchers and the peptide community most often explore NAD+ for — so you know you’re in the right place. They describe what is being studied, not proven benefits, approved uses, or promised results.
Longevity & cellular aging
Investigated for its role in cellular aging, since NAD+ levels decline with age in preclinical and observational research.
Cellular energy metabolism
Studied as a coenzyme central to how cells generate energy, explored in research on metabolism and NAD+ redox pathways.
Mitochondrial function
Researched for its part in mitochondrial redox reactions and energy output, largely in preclinical and animal models.
Cognition & neuroprotection
Explored in research on brain aging and neurodegeneration; investigational and not approved for any such use.
Evidence ranges from early laboratory work to clinical trials depending on the use — the sections below cover the actual data and sources.
Quickstart Highlights
NAD+ (nicotinamide adenine dinucleotide) is a coenzyme found in all living cells — not a peptide. It is central to cellular energy metabolism and acts as a substrate for sirtuins and PARP enzymes involved in DNA repair and aging[1][5]. This educational page outlines a once-daily subcutaneous approach dosed in milligrams, with a dilution chosen so doses land on easy-to-read insulin-syringe marks. Injectable/IV NAD+ is not FDA-approved (or approved by any major regulator) for energy, metabolic or longevity uses, and its safety and efficacy for these uses are not established — presented for research and educational use only.
Add 5.0 mL bacteriostatic water to one 500 mg vial → 100 mg/mL, a convenient dilution where 1 unit = 1 mg on a U-100 syringe.
50–100 mg once daily subcutaneously, starting low and titrating up to tolerance (flushing or GI upset) across an 8–12 week course.
At 100 mg/mL, 1 unit = 1 mg; 50 mg = 50 units (0.50 mL) and 100 mg = 100 units (1.0 mL) on a U-100 syringe.
Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the solution.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
Frequency: one subcutaneous injection each day, starting low (~50 mg) and titrating up to tolerance. Reduce or hold the dose if flushing, nausea or other GI effects occur. NAD+ may also be given intravenously in clinical settings, but that requires professional supervision[3][4]. These figures come from reference protocols, not from approved human dosing.
Reconstitution Steps
Draw 5.0 mL of bacteriostatic water into a sterile syringe.
Release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.
At 100 mg/mL each milligram equals one unit on a U-100 syringe, so dosing is direct and easy to read. Keep the solution refrigerated and protected from light; avoid freezing, since freeze–thaw cycles degrade NAD+ in solution.
Supplies Needed
Quantities below assume an 8–16 week course of once-daily injections with gradual titration.
A 500 mg vial covers roughly 5–10 days at 50–100 mg/day, so plan multiple vials per week depending on your dose.
- 8 weeks (~75 mg/day avg): ~9 vials
- 12 weeks: ~13 vials
- 16 weeks: ~17 vials
- Per injection: 1 syringe
- 8 weeks (once daily): ~56 syringes
- 16 weeks (once daily): ~112 syringes
Use 5.0 mL per 500 mg vial for reconstitution.
- 8 weeks (~9 vials): ~45 mL → 5 bottles
- 16 weeks (~17 vials): ~85 mL → 9 bottles
One for the vial stopper + one for the injection site each day.
- Per injection: 2 swabs
- 8 weeks (once daily): ~112 swabs → 1–2 boxes
Protocol Overview
A concise summary of the once-daily subcutaneous regimen, drawn from commonly cited reference protocols.
- ▪Goal: Support cellular energy metabolism, mitochondrial function and DNA-repair pathways by replenishing NAD+ — goals pursued in research and wellness, not established for injectable NAD+ in humans[5][6].
- ▪Schedule: Daily subcutaneous injections for 8–12 weeks, optionally extended to ~16 weeks for longer research goals.
- ▪Dose Range: 50–100 mg per day subcutaneously, starting low and titrating up to tolerance.
- ▪Reconstitution: 5.0 mL bacteriostatic water per 500 mg vial gives 100 mg/mL (1 unit = 1 mg) for direct measurements.
- ▪Storage: Keep the dry vial frozen at −20 °C (−4 °F); once mixed, refrigerate at 2–8 °C and do not freeze the solution.
Dosing Protocol
A suggested daily subcutaneous titration approach based on common reference doses.
- ▪Start: Begin at 50 mg once daily to gauge tolerability (watch for flushing or GI upset).
- ▪Titrate: Increase by roughly 25 mg every two weeks as tolerated.
- ▪Target: Reach about 100 mg daily by weeks 5–12.
- ▪Cycle Length: Typically 8–12 weeks; some references extend to ~16 weeks.
- ▪Timing: Inject at a consistent time each day and rotate injection sites systematically.
Storage Instructions
Correct storage is what preserves NAD+ stability and activity.
- ▪Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions and limit moisture exposure[7].
- ▪Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F), protected from light, and use within about 14 days; do not freeze the mixed solution, as freeze–thaw degrades NAD+ in solution[8].
- ▪Handling: Let frozen vials warm to room temperature before opening so condensation won’t form, and keep the solution clear of heat and direct light.
- ▪Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.
Important Notes
Practical points that keep daily administration safe and consistent.
- ▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
- ▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[9].
- ▪Slow injection: Push the plunger slowly and pause a few seconds before withdrawing the needle to prevent backflow.
- ▪Recordkeeping: Log the daily dose, injection site and any observations to keep the protocol consistent.
- ▪Regulatory note: Injectable/IV NAD+ is not FDA-approved (or approved by any major regulator) for energy, metabolic or longevity uses; safety and efficacy for these uses are not established[10].
How This Works
NAD+ (nicotinamide adenine dinucleotide) is a coenzyme present in every living cell — not a peptide[1][2]. It is central to the redox reactions that power cellular energy metabolism.
NAD+ shuttles electrons in glycolysis, the TCA cycle and oxidative phosphorylation, making it essential to mitochondrial energy production. It is also a required substrate for sirtuins and PARP enzymes involved in DNA repair, gene regulation and processes tied to aging[5][6].
Cellular NAD+ levels decline with age and metabolic stress, which has prompted interest in raising them through precursors or direct administration for energy, metabolic and longevity goals[11]. Whether injected NAD+ meaningfully restores intracellular levels in humans remains unsettled[12].
Important caveat: injectable and IV NAD+ are not FDA-approved (nor approved by any major regulator) for energy, metabolic or longevity uses, and their safety and efficacy for these marketed uses are not established. Most supporting data are preclinical or small uncontrolled human reports, and the marketed benefits should be read as hypotheses.
NAD+ is a naturally occurring coenzyme, not a peptide and not an approved injectable medicine for these uses. This page is presented for research and educational purposes only.
Lifestyle Factors
Habits that may support NAD+ metabolism and energy alongside the protocol.
- ▪Nutrition: Eat a balanced diet with NAD+ precursors (niacin/vitamin B3, tryptophan) and supporting B-vitamins and magnesium.
- ▪Exercise: Regular resistance and aerobic training naturally upregulates NAD+ biosynthesis and mitochondrial adaptation.
- ▪Sleep: Aim for 7–9 hours to support cellular repair and circadian NAD+ metabolism.
- ▪Alcohol & stress: Limit alcohol (its metabolism consumes NAD+) and manage stress, since chronic stress activates PARPs that deplete NAD+ pools.
Potential Benefits & Side Effects
What preclinical work and uncontrolled human reports describe; rigorous human evidence is limited and individual results vary.
Potential Benefits
- ▪Cellular energy (preclinical): Restoring NAD+ pools is reported to support mitochondrial function and energy metabolism in laboratory models[5][6].
- ▪Metabolism & aging (preclinical): NAD+-dependent sirtuin and PARP activity has been linked to DNA repair and metabolic regulation in animal models[11].
- ▪Tolerability (human reports): Small uncontrolled reports describe NAD+ infusions as generally tolerated, with dose-related flushing and GI effects.
- ▪Note on humans: These benefits are not established in humans — injectable NAD+ is not FDA-approved and lacks adequate controlled efficacy trials[13].
Common Side Effects
- ▪Flushing & GI effects: Flushing, nausea, cramping or lightheadedness can occur, especially if dosed too high or too fast; titrate slowly.
- ▪Injection-site & unknown long-term profile: Mild injection-site reactions can occur, and long-term human safety data are limited, so caution and monitoring are advised.
- ▪Not approved: Injectable/IV NAD+ is not FDA-approved for these uses; quality and dosing of compounded or research material vary.
Injection Technique
General subcutaneous technique, following established clinical best-practice guidance[14][15].
Pre-Injection Preparation
- ▪Wash your hands well with soap and water.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[15].
- ▪Draw the intended dose, then check for air bubbles and push any out.
Injection Procedure
- ▪Pinch a skinfold at the chosen site between thumb and forefinger.
- ▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[14].
- ▪Skip aspiration for subcutaneous shots — it isn’t needed[14].
- ▪Press the plunger slowly and steadily until it’s fully down.
- ▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
Post-Injection Care
- ▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away.
- ▪Rotate the injection site each day to prevent irritation and lipohypertrophy[9].
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
For high-purity research material, we point researchers to Prime Lab Peptides for NAD+ (500 mg).
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
Pharmaceuticals (Basel)Clinical evidence for targeting NAD+ therapeutically: metabolic pathways and therapeutic potential.
- 2
PMC — NAD+ Metabolism ReviewComprehensive review of NAD+ metabolism, mitochondrial function and aging.
- 3
Frontiers in Aging NeurosciencePilot study of human plasma and urine NAD+ metabolome during a 6-hour IV infusion (750 mg).
- 4
Fagron Academy (US)NAD+ dosing review: routes, protocols and case-study insights from clinical practice.
- 5
PMC — NAD+ Pharmacology & ImplicationsPharmacology and potential implications of NAD+ metabolism, aging and therapeutics.
- 6
Sirtuins, PARPs & NAD+ (Review)NAD+ as a substrate for sirtuins and PARPs in DNA repair and aging (review).
- 7
Empower Pharmacy — NAD+ InjectionNAD+ injection (lyophilized): compounding specifications and reconstitution guidance.
- 8
Sigma-Aldrich — NAD+ Product InformationBeta-nicotinamide adenine dinucleotide (NAD+) product specification and stability data.
- 9
NCBI BookshelfBest practices for subcutaneous injection: aseptic technique and site rotation.
- 10
PubMed — NAD+ Clinical Safety ReviewEvaluation of safety and effectiveness of NAD+ across clinical conditions: systematic review.
- 11
Jinfiniti — NAD+ Dosage ChartHuman case reports: benefits at 100–300 mg/day ranges for cognitive and metabolic support.
- 12
Olympia Pharmacy — NAD+ InjectionNAD+ injection (nicotinamide adenine dinucleotide): compounding pharmacy information and dosing guidance.
- 13
AgelessRx — NAD+ Injection OverviewNAD+ injection clinical overview and administration protocols.
- 14
Centers for Disease Control and Prevention (CDC)Subcutaneous injection technique: angle, site and no-aspiration guidance.
- 15
Subcutaneous Injection Technique (Patient Education)How to administer a subcutaneous injection: clinical technique guidelines.
- 16
Prime Lab PeptidesNAD+ (500 mg) product page — purity specifications and certificates of analysis.
NAD+ — frequently asked questions
How do I reconstitute a 500 mg vial of NAD+?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to NAD+?
There is no single correct amount — more water simply spreads the same 500 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store NAD+ after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 500 mg vial of NAD+ provide?
Divide the vial strength of 500 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is NAD+ approved for human use?
No. NAD+ is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
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