Epithalon (10mg Vial) Dosage Protocol
Pineal tetrapeptide studied for telomerase/longevity — research/educational dosing reference.
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers. Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →Mix & measure Epithalon · 10 mg
Dosing & Reconstitution Guide
A single practical dilution with accurate once-daily dosing, step by step
Standard Cycle Approach (2 mL = 5 mg/mL)
Reconstitute: Add 2.0 mL bacteriostatic water to one 10 mg vial → final concentration 5 mg/mL (5,000 mcg/mL).
Typical dose: 5–10 mg once daily over a short ~10–20 day cycle, repeated 2–3× per year.
Easy measuring: At 5 mg/mL, 1 unit = 50 mcg on a U-100 syringe, so 5 mg = 100 units and 10 mg = 200 units — whole, easy-to-read volumes.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the mixed solution.
Why Epithalon draws research interest
These are the directions researchers and the peptide community most often explore Epithalon for — so you know you’re in the right place. They describe what is being studied, not proven benefits, approved uses, or promised results.
Telomere & aging
The primary draw: investigated in Khavinson bioregulator research for telomerase activation and telomere length in cell and animal models.
Longevity & lifespan
Explored in preclinical, animal-only studies (mice, rats, Drosophila) on lifespan and age-related biomarkers; not an approved anti-aging therapy.
Sleep & circadian rhythm
Studied as a pineal peptide for its role in melatonin and circadian regulation, mostly in aging-focused research on older subjects.
Antioxidant & immune modulation
Researched as a bioregulator for markers of oxidative stress and immune function in preclinical models; investigational, not clinically established.
Evidence ranges from early laboratory work to clinical trials depending on the use — the sections below cover the actual data and sources.
Quickstart Highlights
Epithalon (also written Epitalon) is a synthetic tetrapeptide — sequence Ala-Glu-Asp-Gly — modelled on the pineal-gland peptide epithalamin[1][2]. It has been studied — largely in older Russian (Khavinson) research — for telomerase activation, telomere maintenance and circadian/melatonin regulation. This educational page outlines a short subcutaneous cycle with a dilution chosen so doses land on easy-to-read insulin-syringe marks. Epithalon is not approved by the FDA, EMA or any major Western regulator; the evidence is mostly older and preclinical with limited independent replication. It is an unapproved research chemical, presented for research and educational use only.
Add 2.0 mL bacteriostatic water to one 10 mg vial → 5 mg/mL (5,000 mcg/mL), a clean dilution that lands doses on round insulin-syringe marks.
5–10 mg once daily for a short ~10–20 day cycle, typically repeated 2–3× per year.
At 5 mg/mL, 1 unit = 50 mcg; 5 mg = 100 units (1.0 mL) and 10 mg = 200 units (2.0 mL) on a U-100 syringe.
Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the solution.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
Frequency: one subcutaneous injection each day for a short cycle of roughly 10–20 days, commonly repeated 2–3 times per year in cited research schedules[3][4]. These figures come from older reference protocols, not from approved human dosing.
Reconstitution Steps
Draw 2.0 mL of bacteriostatic water into a sterile syringe.
Release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.
The 2.0 mL dilution gives a clean 5 mg/mL concentration, so a 5 mg dose reads at exactly 100 units (1.0 mL) on a U-100 syringe. Avoid freezing the reconstituted solution, since freeze–thaw can denature the peptide.
Supplies Needed
Quantities below assume a short 10–20 day cycle of once-daily injections, repeated a few times per year.
A 10 mg vial covers 1–2 days at 5–10 mg/day, so a single ~10–20 day cycle needs several vials.
- 10-day cycle (5 mg/day): ~5 vials
- 20-day cycle (5 mg/day): ~10 vials
- 20-day cycle (10 mg/day): ~20 vials
- Per injection: 1 syringe
- 10-day cycle: ~10 syringes
- 20-day cycle: ~20 syringes
Use ~2.0 mL per 10 mg vial for reconstitution.
- 10-day cycle (5 vials): ~10 mL → 1 bottle
- 20-day cycle (10 vials): ~20 mL → 2 bottles
One for the vial stopper + one for the injection site each day.
- Per injection: 2 swabs
- 20-day cycle (once daily): ~40 swabs → 1 box
Protocol Overview
A concise summary of the short cycled regimen, drawn from older cited reference protocols.
- ▪Goal: Studied for telomerase activation, telomere maintenance and circadian/melatonin regulation — effects reported mostly in older Russian and preclinical work, not established in humans[5][6].
- ▪Schedule: Daily subcutaneous injections for a short ~10–20 day cycle, typically repeated 2–3 times per year.
- ▪Dose Range: 5–10 mg per day for the duration of the short cycle.
- ▪Reconstitution: 2.0 mL bacteriostatic water per 10 mg vial gives 5 mg/mL for accurate unit measurements.
- ▪Storage: Keep the dry vial frozen at −20 °C (−4 °F); once mixed, refrigerate at 2–8 °C and do not freeze the solution.
Dosing Protocol
A suggested short-cycle approach based on older reference doses.
- ▪Start: Begin at 5 mg once daily to gauge tolerability.
- ▪Hold or extend: Maintain 5 mg/day, or extend toward 10 mg/day for the optional days 11–20 window if tolerated.
- ▪Target: A cumulative cycle dose in the range of roughly 50–100 mg across the 10–20 day course.
- ▪Cycle Length: Typically ~10–20 days, repeated 2–3 times per year.
- ▪Timing: Inject at a consistent time each day and rotate injection sites systematically.
Storage Instructions
Correct storage is what preserves the peptide’s stability and activity.
- ▪Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions and limit moisture exposure[7].
- ▪Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F) and use within about 28 days; do not freeze the mixed solution, as freezing can denature peptides[8].
- ▪Handling: Let frozen vials warm to room temperature before opening so condensation won’t form, and keep the solution clear of heat and direct light.
- ▪Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.
Important Notes
Practical points that keep daily administration safe and consistent.
Further reading: which peptides have real human sleep data puts Epithalon’s melatonin research next to DSIP, Ipamorelin and Selank, and shows what each study actually measured.
- ▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
- ▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[9].
- ▪Slow injection: Push the plunger slowly and pause a few seconds before withdrawing the needle to prevent backflow.
- ▪Recordkeeping: Log the daily dose, injection site and any observations to keep the protocol consistent.
- ▪Regulatory note: Epithalon is not approved by the FDA, EMA or any major Western regulator for human use, and its evidence base is limited and largely unreplicated outside older Russian research[10].
How This Works
Epithalon (Epitalon) is a synthetic tetrapeptide with the sequence Ala-Glu-Asp-Gly (AEDG), designed as a short analogue of the natural pineal-gland peptide epithalamin[1][2]. The pineal peptides it is modelled on are involved in circadian and neuroendocrine regulation.
Its most-discussed proposed mechanism is telomerase activation — the enzyme that maintains telomeres at the ends of chromosomes. In cell-culture and animal work from the Khavinson group, Epithalon has been reported to induce telomerase expression, support telomere maintenance, and influence gene expression[5][6]. These findings come largely from older Russian research.
It has also been studied for circadian and melatonin regulation — restoring more youthful melatonin rhythms in aged animals — and, in long-term rodent studies, for effects framed as longevity / anti-aging[11]. Such longevity claims should be read cautiously as hypotheses, not established outcomes.
Important caveat: the human evidence base is thin and largely unreplicated outside the original Russian groups, and most data are preclinical or older clinical work without modern independent confirmation[13]. Anti-aging and telomere claims rest on this limited literature and should not be overstated.
Epithalon is not approved by the FDA, EMA or any major Western regulator. It is an unapproved research chemical presented here for research and educational purposes only.
Lifestyle Factors
Habits that support general healthy aging alongside the protocol.
- ▪Nutrition: A balanced, antioxidant-rich diet supports general cellular health during a cycle.
- ▪Activity & rest: Pair regular activity with consistent recovery; chronic overtraining works against healthy aging.
- ▪Sleep: Aim for 7–9 hours; consistent sleep reinforces the circadian rhythms Epithalon is studied for.
- ▪Stress: Manage stress with evidence-based practices, since it influences overall cellular aging.
Potential Benefits & Side Effects
What older preclinical and Russian research describe; human evidence is limited and largely unreplicated.
Potential Benefits
- ▪Telomerase & telomeres (preclinical): Cell and animal studies report telomerase activation and telomere maintenance[5][6].
- ▪Circadian / melatonin (preclinical): Reported to help restore melatonin rhythms and circadian regulation in aged animals[11].
- ▪Longevity signals (preclinical): Some long-term rodent studies describe lifespan-related effects — framed as hypotheses, not proven benefits.
- ▪Note on humans: These benefits are not established in humans — the evidence is mostly older Russian and preclinical work with limited independent replication[13].
Common Side Effects
- ▪Injection-site reactions: Mild redness, tenderness or soreness can occur; rotating sites helps.
- ▪Unknown long-term profile: Human safety data is limited, so caution and monitoring are advised.
- ▪Unapproved status: Epithalon is not approved by any major Western regulator; quality and long-term safety are not independently assured.
Injection Technique
General subcutaneous technique, following established clinical best-practice guidance[14][15].
Pre-Injection Preparation
- ▪Wash your hands well with soap and water.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[15].
- ▪Draw the intended dose, then check for air bubbles and push any out.
Injection Procedure
- ▪Pinch a skinfold at the chosen site between thumb and forefinger.
- ▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[14].
- ▪Skip aspiration for subcutaneous shots — it isn’t needed[14].
- ▪Press the plunger slowly and steadily until it’s fully down.
- ▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
Post-Injection Care
- ▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away.
- ▪Rotate the injection site each day to prevent irritation and lipohypertrophy[9].
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
For high-purity research peptides, we point researchers to Prime Lab Peptides. Note: Epithalon is not currently listed there, so the link below points to their main catalog.
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
Khavinson V.Kh. et al. (peptide bioregulators)Foundational work describing short pineal peptides (epithalamin) and the synthetic AEDG tetrapeptide Epithalon.
- 2
Bulletin of Experimental Biology and MedicineReports on the synthesis and biological activity of the Ala-Glu-Asp-Gly (Epitalon) tetrapeptide.
- 3
Neuro Endocrinology LettersCited Russian clinical/experimental protocols describing short cycled courses of Epithalon.
- 4
Advances in Gerontology (Russian)Reference gerontology protocols and cycled dosing schedules for pineal peptide preparations.
- 5
Khavinson V.Kh., Bondarev I.E., et al. (telomerase)Cell-culture work reporting telomerase induction and telomere elongation by the AEDG peptide.
- 6
BiogerontologyStudies on gene-expression and telomere-related effects attributed to Epithalon in model systems.
- 7
Peptide handling / lyophilized storage guidanceGeneral guidance on storing lyophilized research peptides cold, dry and dark.
- 8
Peptide reconstitution & stabilityOn reconstituting peptides with bacteriostatic water and avoiding freeze-thaw of solutions.
- 9
Injection-site rotation guidanceClinical guidance on rotating subcutaneous injection sites to limit irritation and lipohypertrophy.
- 10
Regulatory status (FDA/EMA)Epithalon is not an approved drug with any major Western regulator; it is an unapproved research chemical.
- 11
Experimental GerontologyRodent studies describing melatonin/circadian restoration and lifespan-related effects of pineal peptides.
- 12
Peptide pharmacology reviewBackground on short peptide bioregulators and their proposed neuroendocrine mechanisms.
- 13
Independent replication / evidence appraisalNotes that Epithalon human data are limited and largely unreplicated outside the original Russian groups.
- 14
Centers for Disease Control and Prevention (CDC)Subcutaneous injection technique: angle, site and no-aspiration guidance.
- 15
Subcutaneous Injection Technique (Patient Education)How to administer a subcutaneous injection: clinical technique guidelines.
- 16
Prime Lab PeptidesResearch-peptide supplier main catalog — purity specifications and certificates of analysis (Epithalon not currently listed).
Epithalon — frequently asked questions
How do I reconstitute a 10 mg vial of Epithalon?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to Epithalon?
There is no single correct amount — more water simply spreads the same 10 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store Epithalon after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 10 mg vial of Epithalon provide?
Divide the vial strength of 10 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is Epithalon approved for human use?
No. Epithalon is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
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