5-Amino-1MQ
Small molecule enzyme inhibitor that boosts cellular metabolism by blocking NNMT, increasing NAD⁺ levels and energy expenditure to promote fat loss without appetite suppression.
Note: 5-Amino-1MQ is not a peptide — it’s a small organic molecule (quinolinium analog). It’s often sold alongside peptides due to its metabolic research applications, but chemically it’s a distinct compound class.
⚡ Executive Summary
5-Amino-1MQ is a selective NNMT inhibitor that boosts NAD⁺ levels and cellular energy expenditure. In mouse studies, it produced significant fat loss without reducing food intake, improved insulin sensitivity, and lowered cholesterol. It remains an experimental research compound with no human trials yet — not FDA approved for any indication.
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Overview
⚗️ What is 5-Amino-1MQ?
5-Amino-1MQ (5-amino-1-methylquinolinium) is a small cationic molecule designed to shut down NNMT’s activity.
By binding to NNMT’s active site, it prevents the enzyme from methylating nicotinamide, allowing more NAD⁺ to be recycled for cellular energy.
🎯 Key Features
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🔬
Selective NNMT inhibitor — no off-target effects
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⚡
Boosts NAD⁺ — increases cellular metabolism
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🔥
Burns fat — without suppressing appetite
Research only: 5-Amino-1MQ has no published human clinical trials. All benefits are based on mouse studies. It is not FDA-approved for any indication.
Entity Properties
| Aliases | 5-Amino-1MQ, 5-Amino-1-methylquinolinium iodide |
|---|---|
| Type | Small molecule (quinolinium analog) — not a peptide |
| Molecular Weight | 286.11 Da (iodide salt) |
| CAS Number | 42464-96-0 |
| Target | NNMT (Nicotinamide N-methyltransferase) |
| Pathway | NAD⁺ salvage pathway / cellular metabolism |
| Diluent(s) | Bacteriostatic water or sterile saline |
| Concentration | e.g. 10 mg in 2 mL = 5 mg/mL solution |
| Storage (dry) | Room temp or refrigerate; stable for months |
| Storage (solution) | Refrigerate 2–8°C; use within ~30 days |
Mechanism of Action
🧠 How does 5-Amino-1MQ work?
NNMT acts like a metabolic brake — it methylates and consumes nicotinamide, reducing the building blocks available to recycle NAD⁺. High NNMT activity lowers NAD⁺ levels and slows cellular energy processes.
5-Amino-1MQ blocks NNMT, so nicotinamide isn’t wasted and can instead be recycled into NAD⁺. With more NAD⁺ available, cells ramp up their metabolic processes — burning more fat and storing less energy as adipose tissue.
🔒 Step 1: Inhibition
5-Amino-1MQ binds to NNMT’s active site, blocking the enzyme from consuming nicotinamide
⚡ Step 2: NAD⁺ Boost
Nicotinamide is recycled into NAD⁺ instead of being wasted as MNA → cellular NAD⁺ levels increase
🔥 Step 3: Metabolism↑
Higher NAD⁺ → increased fuel oxidation, more fat burning, less fat storage, higher energy expenditure
Why is this unique? Unlike appetite suppressants, 5-Amino-1MQ works within cells to change how fuel is used. The body burns more calories at rest without eating less.
Research Benefits
🐭 Preclinical Findings (Mouse Studies)
Fat Loss
Significant reductions in body weight and white fat mass without eating less — genuine metabolic fat-burning
Insulin Sensitivity
Better glucose tolerance and normalized blood sugar, reversing diet-induced T2D features
Cholesterol
Lower plasma cholesterol levels indicating favorable shift in lipid metabolism
Thermogenesis
Increased oxygen consumption in white adipose tissue — more calories burned as heat
Muscle Regeneration
Activated senescent muscle stem cells in aged mice, improving regenerative capacity
Safety Profile
No observable adverse effects even at high doses (up to 60 mg/kg/day) — wide safety margin
Important: All benefits are from preclinical (mouse) studies. Human outcomes might differ. Rigorous clinical trials are needed to confirm efficacy and safety in people.
Dosage & Administration
Research use only. No official human dose guidelines exist. The following is educational based on preclinical data and experimental protocols.
📊 Dosing Reference
| Mouse Dose | ~20 mg/kg/day (effective in studies) |
|---|---|
| Experimental Human | ~50–100 mg/day (unofficial, extrapolated) |
| Route | Subcutaneous injection (oral not effective) |
| Frequency | Once or twice daily |
| Cycle Length | 4–12 weeks (unofficial protocols) |
Reconstitute
Add bacteriostatic water to vial (e.g., 2 mL to 10 mg = 5 mg/mL)
Calculate Dose
Double-check math; at 5 mg/mL, 50 mg = 10 units (1 mL)
Inject Sub-Q
Subcutaneous into abdomen, thigh, or shoulder with insulin syringe
Store Properly
Refrigerate solution (2–8°C); use within 30 days
Comparison
How does 5-Amino-1MQ compare to other weight management compounds? It has a unique metabolic mechanism distinct from appetite suppressants or hormone mimetics.
5-Amino-1MQ
No appetite suppression — burns fat by increasing cellular energy expenditure. Mice ate normally but lost weight.
Semaglutide (GLP-1)
Strong appetite suppression — patients feel full sooner and eat much less. Can cause nausea. FDA-approved.
AOD-9604
No appetite effect — activates fat breakdown via β3 receptors. Modest results in trials (~2.8 kg in 12 weeks).
Key difference: 5-Amino-1MQ represents a metabolic approach — making the body burn more energy rather than causing people to eat less. It could theoretically complement appetite-based therapies.
FAQ
Bottom line: 5-Amino-1MQ is a promising research compound that boosts metabolism through a novel NNMT inhibition mechanism. Mouse studies show impressive fat loss without appetite suppression. However, it remains experimental with no human data — treat it as “potential in a vial” until clinical trials validate its effects in people.