TB-500 (5mg Vial) Dosage Protocol
TB-500 is a synthetic peptide fragment that copies the active region (Ac-LKKTETQ) of Thymosin Beta-4, a naturally occurring protein involved in cell migration and tissue repair. It is referenced as a once-daily subcutaneous injection dosed in micrograms with gradual titration. There are no completed human efficacy trials of the TB-500 fragment; presented for research and educational purposes only.
Synthetic fragment of Thymosin Beta-4; preclinically linked to actin regulation, cell migration and angiogenesis. No completed human efficacy trials of the fragment.
Once-daily subcutaneous injection, titrated from ~500 mcg to ~1000 mcg/day (about ~5 mg per week).
3 mL bacteriostatic water per 5 mg vial gives ~1.67 mg/mL for accurate insulin-syringe dosing.
Freeze the lyophilized vial; refrigerate the reconstituted solution and do not freeze it.
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers. Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →Mix & measure TB-500 · 5 mg
Dosing & Reconstitution Guide
A single practical dilution with accurate once-daily dosing, step by step
Standard / Gradual Approach (3 mL = ~1.67 mg/mL)
Reconstitute: Add 3.0 mL bacteriostatic water to one 5 mg vial → final concentration ~1.67 mg/mL (1,667 mcg/mL).
Typical daily range: 500–1000 mcg once daily, raised gradually over an 8–12 week course.
Easy measuring: At ~1.67 mg/mL, 1 unit ≈ 16.7 mcg on a U-100 syringe. Using the large 3.0 mL dilution keeps every dose at 30 units or more for excellent measuring accuracy.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the mixed solution.
Why TB-500 draws research interest
These are the directions researchers and the peptide community most often explore TB-500 for — so you know you’re in the right place. They describe what is being studied, not proven benefits, approved uses, or promised results.
Tissue repair & wound healing
Investigated in preclinical research on how the Ac-LKKTETQ region promotes cell migration and tissue regeneration.
Injury & muscle recovery
Explored in animal studies for its role in soft-tissue, tendon, and muscle repair after injury; not approved for human use.
Anti-inflammatory activity
Studied in preclinical models for how Thymosin Beta-4 fragments may modulate inflammation during the healing response.
Angiogenesis & cell migration
Researched as a regulator of new blood-vessel formation and cell movement in early-stage laboratory work; investigational only.
Evidence ranges from early laboratory work to clinical trials depending on the use — the sections below cover the actual data and sources.
Quickstart Highlights
TB-500 is a lab-made peptide fragment that copies the active region (the sequence Ac-LKKTETQ) of Thymosin Beta-4, a naturally occurring 43-amino-acid protein tied to cell movement and tissue repair[1][5]. This educational page outlines a once-daily subcutaneous approach with a dilution chosen so doses land on easy-to-read insulin-syringe marks. It is an unapproved research chemical, not a medicine, and there are no completed human efficacy trials of the TB-500 fragment itself — presented for research and educational use only.
Add 3.0 mL bacteriostatic water to one 5 mg vial → ~1.67 mg/mL (1,667 mcg/mL), the largest practical dilution for accurate dosing.
500–1000 mcg once daily, titrated upward gradually across an 8–12 week course (about ~5 mg per week on average).
At ~1.67 mg/mL, 1 unit ≈ 16.7 mcg; 500 mcg ≈ 30 units and 1000 mcg ≈ 60 units on a U-100 syringe.
Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the solution.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
Frequency: one subcutaneous injection each day, titrating up as tolerated. Across the cycle the weekly total averages roughly 5 mg, in line with commonly cited research schedules[3][4]. These figures come from reference protocols, not from approved human dosing.
Reconstitution Steps
Draw 3.0 mL of bacteriostatic water into a sterile syringe.
Release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.
The 3.0 mL dilution is deliberately large so each dose reads at 30 units or more, where U-100 syringe markings are most precise. Avoid freezing the reconstituted solution, since freeze–thaw can denature the peptide.
Supplies Needed
Quantities below assume an 8–16 week course of once-daily injections with gradual titration.
A 5 mg vial is roughly one week of dosing at the ~5 mg/week average, so plan about one vial per week.
- 8 weeks: ~8 vials
- 12 weeks: ~12 vials
- 16 weeks: ~16 vials
- Per injection: 1 syringe
- 8 weeks (once daily): ~56 syringes
- 16 weeks (once daily): ~112 syringes
Use ~3.0 mL per 5 mg vial for reconstitution.
- 8 weeks (8 vials): ~24 mL → 3 bottles
- 16 weeks (16 vials): ~48 mL → 5 bottles
One for the vial stopper + one for the injection site each day.
- Per injection: 2 swabs
- 8 weeks (once daily): ~112 swabs → 1–2 boxes
Protocol Overview
A concise summary of the once-daily regimen, drawn from commonly cited reference protocols.
- ▪Goal: Support tissue repair, wound healing and angiogenesis via the active Thymosin Beta-4 fragment — effects reported preclinically, not established in humans[5][6].
- ▪Schedule: Daily subcutaneous injections for 8–12 weeks, optionally extended to ~16 weeks for longer research goals.
- ▪Dose Range: 500–1000 mcg per day with gradual titration, averaging about ~5 mg per week.
- ▪Reconstitution: 3.0 mL bacteriostatic water per 5 mg vial gives ~1.67 mg/mL for accurate unit measurements.
- ▪Storage: Keep the dry vial frozen at −20 °C (−4 °F); once mixed, refrigerate at 2–8 °C and do not freeze the solution.
Dosing Protocol
A suggested daily titration approach based on common reference doses.
- ▪Start: Begin at 500 mcg once daily to gauge tolerability.
- ▪Titrate: Increase by roughly 100–150 mcg every two weeks as tolerated.
- ▪Target: Reach about 750–1000 mcg daily by weeks 5–12.
- ▪Cycle Length: Typically 8–12 weeks; some references extend to ~16 weeks.
- ▪Timing: Inject at a consistent time each day and rotate injection sites systematically.
Storage Instructions
Correct storage is what preserves the peptide’s stability and activity.
- ▪Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions and limit moisture exposure[7].
- ▪Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F) and use within about 28 days; do not freeze the mixed solution, as freezing can denature peptides[8].
- ▪Handling: Let frozen vials warm to room temperature before opening so condensation won’t form, and keep the solution clear of heat and direct light.
- ▪Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.
Important Notes
Practical points that keep daily administration safe and consistent.
- ▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
- ▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[9].
- ▪Slow injection: Push the plunger slowly and pause a few seconds before withdrawing the needle to prevent backflow.
- ▪Recordkeeping: Log the daily dose, injection site and any observations to keep the protocol consistent.
- ▪Regulatory note: TB-500 is prohibited by WADA for athletic use and is not FDA-approved for human administration[10].
How This Works
TB-500 is a synthetic fragment that reproduces the active N-terminal region of Thymosin Beta-4 (Tβ4) — specifically the short sequence Ac-LKKTETQ[1][2]. That region carries the actin-binding activity of the full protein.
Its core proposed mechanism is the sequestering of G-actin, which helps regulate actin polymerization — the process behind the cell migration that is central to wound healing. In preclinical work the fragment has also been linked to angiogenesis (new blood-vessel growth, including VEGF signalling), recruitment of stem and progenitor cells, and reduced inflammation and fibrosis[5][6].
Animal studies have described greater collagen deposition and shorter healing times at injury sites treated with thymosin fragments[11], and some metabolic work suggests TB-500 may behave as a prodrug that cleaves to a shorter active metabolite[12].
Important caveat: there are no completed human efficacy trials of the TB-500 fragment itself. The limited human data involve full-length Thymosin Beta-4 — a different, larger molecule, studied mainly as an eye-drop — not this peptide. Musculoskeletal “recovery” and “healing” claims rest on animal data and anecdote, and should be read as hypotheses.
TB-500 is not an approved medicine. It is an unapproved research chemical presented here for research and educational purposes only.
Lifestyle Factors
Habits that may support recovery alongside the protocol.
- ▪Nutrition: Keep protein intake adequate to give tissue repair the building blocks it needs.
- ▪Activity & rest: Pair appropriate movement with real recovery time and avoid overtraining during an injury-recovery phase.
- ▪Sleep: Aim for 7–9 hours to support the body’s natural repair processes.
- ▪Stress: Manage stress with evidence-based practices, since it influences overall healing.
Potential Benefits & Side Effects
What preclinical and veterinary literature describe; human evidence is limited and individual results vary.
Potential Benefits
- ▪Wound healing (preclinical): Animal studies report faster wound healing and tissue repair via enhanced angiogenesis and cell migration[5][6].
- ▪Inflammation & fibrosis (preclinical): Thymosin pathways have been linked to reduced inflammation and fibrosis in animal models[11].
- ▪Tolerability (veterinary): Generally well tolerated in veterinary studies, with occasional mild injection-site reactions.
- ▪Note on humans: These benefits are not established in humans — no large-scale clinical trials of the TB-500 fragment have been completed[13].
Common Side Effects
- ▪Injection-site reactions: Mild redness, tenderness or soreness can occur; rotating sites helps.
- ▪Unknown long-term profile: Human safety data is limited, so caution and monitoring are advised.
- ▪Sport restriction: Note that TB-500 is a WADA-prohibited substance for athletes.
Injection Technique
General subcutaneous technique, following established clinical best-practice guidance[14][15].
Pre-Injection Preparation
- ▪Wash your hands well with soap and water.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[15].
- ▪Draw the intended dose, then check for air bubbles and push any out.
Injection Procedure
- ▪Pinch a skinfold at the chosen site between thumb and forefinger.
- ▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[14].
- ▪Skip aspiration for subcutaneous shots — it isn’t needed[14].
- ▪Press the plunger slowly and steadily until it’s fully down.
- ▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
Post-Injection Care
- ▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away.
- ▪Rotate the injection site each day to prevent irritation and lipohypertrophy[9].
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
For high-purity research peptides, we point researchers to Prime Lab Peptides for TB-500 (5 mg).
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
FASEB JournalBiological activities of thymosin beta-4 mapped to active peptide sequences, including the fragment marketed as TB-500.
- 2
Journal of Chromatography A (PubMed)Doping-control analysis of TB-500 as a synthetic thymosin beta-4 fragment in biological samples.
- 3
WADA Scientific ResearchInvestigation of TB-500 metabolism, synthesis of its metabolites, and detection limits.
- 4
Racing Medication & Testing ConsortiumThymosin beta-4 regulatory bulletin covering TB-500 use in equine sports medicine.
- 5
Journal of Investigative Dermatology (PubMed)Thymosin beta-4 accelerates wound healing in a preclinical model.
- 6
FASEB Journal (PubMed)Active-site mapping of thymosin beta-4 fragments for angiogenesis and cell migration.
- 7
Peptide Storage GuideBest practices for storing lyophilized peptides (temperature, humidity and light protection).
- 8
Bacteriostatic Water GuidanceBacteriostatic water for injection: multi-dose vial stability and handling.
- 9
NCBI BookshelfBest practices for subcutaneous injection: aseptic technique and site rotation.
- 10
WADA Prohibited ListClassification of TB-500 as a prohibited substance in competitive sport.
- 11
Journal of Investigative DermatologyThymosin beta-4 wound-healing mechanisms: collagen deposition, angiogenesis and granulation tissue.
- 12
Journal of Chromatography B (PubMed)Quantification of TB-500 metabolites and screening of wound-healing activity (prodrug hypothesis).
- 13
ClinicalTrials.govTrial registry for full-length thymosin beta-4 (e.g., dermal and ocular indications); no completed efficacy trials of the TB-500 fragment.
- 14
Centers for Disease Control and Prevention (CDC)Subcutaneous injection technique: angle, site and no-aspiration guidance.
- 15
Subcutaneous Injection Technique (Patient Education)How to administer a subcutaneous injection: clinical technique guidelines.
- 16
Prime Lab PeptidesTB-500 (5 mg) product page — purity specifications and certificates of analysis.
TB-500 — frequently asked questions
How do I reconstitute a 5 mg vial of TB-500?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to TB-500?
There is no single correct amount — more water simply spreads the same 5 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store TB-500 after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 5 mg vial of TB-500 provide?
Divide the vial strength of 5 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is TB-500 approved for human use?
No. TB-500 is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
New protocols & dosing updates
Reconstitution charts, new peptide protocols and safety updates — straight to your inbox. No spam, unsubscribe anytime.