Selank (5mg Vial) Dosage Protocol
Anxiolytic / nootropic heptapeptide (tuftsin analog) — research/educational dosing reference.
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers. Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →Mix & measure Selank · 5 mg
Dosing & Reconstitution Guide
A single practical dilution with accurate once-daily dosing, step by step
Standard / Gradual Approach (3 mL = ~1.67 mg/mL)
Reconstitute: Add 3.0 mL bacteriostatic water to one 5 mg vial → final concentration ~1.67 mg/mL (1,667 mcg/mL).
Typical daily range: 300–500 mcg once daily, raised gradually over a 4-week cycle (often run 4 weeks on, 4 weeks off).
Easy measuring: At ~1.67 mg/mL, 1 unit ≈ 16.7 mcg on a U-100 syringe; the typical 300–500 mcg dose reads at 18–30 units, comfortably within the precise range of U-100 markings.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the mixed solution.
Why Selank draws research interest
These are the directions researchers and the peptide community most often explore Selank for — so you know you’re in the right place. They describe what is being studied, not proven benefits, approved uses, or promised results.
Anxiety / anxiolytic
Studied in Russian clinical research as an investigational anxiolytic for generalized anxiety; not FDA-approved.
Cognition / nootropic
Investigated in animal and limited human research for effects on attention, memory and mental performance.
Immune modulation
Explored in preclinical research as a tuftsin analog for immune-modulating and interferon-inducing activity.
Neuroprotection / BDNF
Researched in animal models for effects on BDNF expression and monoamine and enkephalin signaling in the brain.
Evidence ranges from early laboratory work to clinical trials depending on the use — the sections below cover the actual data and sources.
Quickstart Highlights
Selank is a synthetic heptapeptide (the sequence Thr-Lys-Pro-Arg-Pro-Gly-Pro), an analog of the immunopeptide tuftsin, studied as an anxiolytic and nootropic agent[1][3]. This educational page outlines a once-daily subcutaneous approach with a dilution chosen so doses land on easy-to-read insulin-syringe marks. Note that the clinically validated, approved route is the intranasal spray (registered only in Russia/Ukraine); in the US, EU and UK Selank is not approved and is sold only as a research chemical — presented for research and educational use only.
Add 3.0 mL bacteriostatic water to one 5 mg vial → ~1.67 mg/mL (1,667 mcg/mL), the largest practical dilution for accurate dosing.
300–500 mcg once daily, titrated upward gradually across a 4-week cycle (commonly run as 4 weeks on, 4 weeks off).
At ~1.67 mg/mL, 1 unit ≈ 16.7 mcg; 300 mcg ≈ 18 units and 500 mcg ≈ 30 units on a U-100 syringe.
Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the solution.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
Frequency: one subcutaneous injection each day, titrating up as tolerated. A common community pattern is a 4-week cycle (or 5 days on / 2 days off) followed by a break[3]. Note that the clinically studied, approved route for Selank is intranasal; subcutaneous injection is a research/community practice with weak pharmacologic rationale, since the peptide’s plasma half-life is only minutes[4]. These figures come from reference protocols, not from approved human dosing.
Reconstitution Steps
Draw 3.0 mL of bacteriostatic water into a sterile syringe.
Release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.
The 3.0 mL dilution is deliberately large so each daily dose reads at roughly 18–30 units, where U-100 syringe markings are most precise. Avoid freezing the reconstituted solution, since freeze–thaw can denature the peptide.
Supplies Needed
Quantities below assume an 8–16 week course of once-daily injections with gradual titration.
At 300–500 mcg per day a 5 mg vial lasts well over a week, but allowing for 4-week cycles and waste, plan for roughly one vial every 1–2 weeks.
- 8 weeks: ~5 vials
- 12 weeks: ~7 vials
- 16 weeks: ~10 vials
- Per injection: 1 syringe
- 8 weeks (continuous use): ~56 syringes
- 16 weeks (continuous use): ~112 syringes
Use ~3.0 mL per 5 mg vial for reconstitution.
- 8 weeks (5 vials): ~15 mL → 2 bottles
- 16 weeks (10 vials): ~30 mL → 3 bottles
One for the vial stopper + one for the injection site each day.
- Per injection: 2 swabs
- 8 weeks (once daily): ~112 swabs → 1–2 boxes
Protocol Overview
A concise summary of the once-daily regimen, drawn from commonly cited reference protocols.
- ▪Goal: Explore Selank’s reported anxiolytic and anti-asthenic (energy-supporting) effects — demonstrated mainly with the intranasal formulation in small Russian trials, where it was comparable to medazepam without sedation or dependence[1][2].
- ▪Schedule: Once-daily subcutaneous injections in 4-week cycles (commonly 4 weeks on, 4 weeks off), repeated as needed.
- ▪Dose Range: 300–500 mcg per day with gradual titration over the cycle.
- ▪Reconstitution: 3.0 mL bacteriostatic water per 5 mg vial gives ~1.67 mg/mL for accurate unit measurements.
- ▪Storage: Keep the dry vial frozen at −20 °C (−4 °F); once mixed, refrigerate at 2–8 °C and do not freeze the solution.
Dosing Protocol
A suggested daily titration approach based on common reference doses.
- ▪Start: Begin at 300 mcg once daily for the first two weeks to gauge tolerability.
- ▪Titrate: Increase to 500 mcg once daily from week 3 if the lower dose is well tolerated.
- ▪Target: Maintain around 500 mcg daily for the remainder of the 4-week cycle.
- ▪Cycle Length: Typically a 4-week cycle followed by a 4-week break; repeat as needed.
- ▪Timing: Inject at a consistent time each day and rotate injection sites systematically.
Storage Instructions
Correct storage is what preserves the peptide’s stability and activity.
- ▪Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions and limit moisture exposure[7].
- ▪Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F) and use within about 28 days; do not freeze the mixed solution, as freezing can denature peptides[8].
- ▪Handling: Let frozen vials warm to room temperature before opening so condensation won’t form, and keep the solution clear of heat and direct light.
- ▪Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.
Important Notes
Practical points that keep daily administration safe and consistent.
- ▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
- ▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[9].
- ▪Slow injection: Push the plunger slowly and pause a few seconds before withdrawing the needle to prevent backflow.
- ▪Recordkeeping: Log the daily dose, injection site and any observations to keep the protocol consistent.
- ▪Regulatory note: Selank is an approved prescription nasal spray only in Russia and Ukraine; in the US, EU and UK it is not approved for human use and is sold strictly as a research chemical[6].
How This Works
Selank is a synthetic heptapeptide with the sequence Thr-Lys-Pro-Arg-Pro-Gly-Pro — an analog of the immunomodulatory peptide tuftsin, modified to resist rapid breakdown[1][3]. It was developed in Russia and is studied mainly as an anxiolytic (anti-anxiety) and anti-asthenic agent.
Its proposed mechanism is unusual: Selank appears to modulate the expression of GABA-A receptor genes without binding the benzodiazepine site, which is offered as an explanation for anxiolytic activity that comes without sedation, tolerance, dependence or withdrawal[3]. It has also been reported to influence serotonin and other monoamines, to inhibit enkephalinase (slowing the breakdown of enkephalins), and in animal work to raise BDNF expression[5].
The strongest human evidence is for the intranasal route: small Russian clinical trials in generalized anxiety and neurasthenia reported anxiolytic effects comparable to medazepam, but without the sedation or dependence typical of benzodiazepines[1][2]. Most cognitive, mood and BDNF findings come from animal studies and should be read as preliminary rather than established in humans.
Important route caveat: the clinically validated and approved formulation is a ready-made intranasal spray. Subcutaneous injection, presented here, is a research/community practice with weak pharmacologic rationale — Selank’s plasma half-life is only minutes[4], so injected dosing has not been validated against the intranasal data.
Approval status: Selank is an approved prescription drug (intranasal) only in Russia and Ukraine for anxiety/GAD. In the US, EU and UK it is not approved and is sold solely as a research chemical, presented here for research and educational purposes only.
Lifestyle Factors
Habits that may support mood, stress resilience and cognition alongside the protocol.
- ▪Nutrition: Eat adequate protein and omega-3 fats to supply the building blocks for neurotransmitter synthesis.
- ▪Activity: Regular aerobic and resistance exercise supports mood regulation and stress resilience.
- ▪Sleep: Aim for 7–9 hours of quality sleep to support neurological function and stress recovery.
- ▪Stress management: Use mindfulness, meditation or breathwork to complement Selank’s anxiolytic effects; minimize alcohol and stimulants.
Potential Benefits & Side Effects
What small human trials and preclinical studies describe; most human data uses the intranasal route, and individual results vary.
Potential Benefits
- ▪Anxiolytic (human, intranasal): Small Russian trials report anxiety reduction comparable to medazepam, without sedation or dependence[1][2].
- ▪Anti-asthenic (human): A 14-day intranasal course in neurasthenia was well tolerated and reported energy-supporting, anti-asthenic effects[2].
- ▪Nootropic signals (animal): Animal studies report effects on serotonin, monoamines and BDNF; cognitive/mood claims are largely preclinical and not established in humans[5].
- ▪Evidence limits: Human evidence is limited to small Russian trials using the intranasal spray; no large-scale or injection-route efficacy trials have been completed[3].
Common Side Effects
- ▪Injection-site reactions: Mild redness, tenderness or soreness can occur; rotating sites helps.
- ▪Unknown long-term profile: Human safety data is limited, so caution and monitoring are advised.
- ▪Stimulating effect: Some users report mild over-stimulation or difficulty sleeping if dosed late in the day; prefer morning administration.
Injection Technique
General subcutaneous technique, following established clinical best-practice guidance[10][11].
Pre-Injection Preparation
- ▪Wash your hands well with soap and water.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[11].
- ▪Draw the intended dose, then check for air bubbles and push any out.
Injection Procedure
- ▪Pinch a skinfold at the chosen site between thumb and forefinger.
- ▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[10].
- ▪Skip aspiration for subcutaneous shots — it isn’t needed[10].
- ▪Press the plunger slowly and steadily until it’s fully down.
- ▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
Post-Injection Care
- ▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away.
- ▪Rotate the injection site each day to prevent irritation and lipohypertrophy[9].
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
For high-purity research peptides, we point researchers to Prime Lab Peptides for Selank (5 mg).
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
PubMed (Zozulya et al.)Efficacy and safety of intranasal Selank in generalized anxiety disorder — randomized human clinical trial.
- 2
PubMed (Medvedev et al.)Selank in neurasthenia: a 14-day intranasal course showing anxiolytic and anti-asthenic effects (human study).
- 3
Hindawi / BioMed Research InternationalReview: intranasal Selank administration — neurobehavioral effects, pharmacology and clinical applications.
- 4
DrugBank / PharmacologySelank pharmacokinetics: rapid plasma degradation (half-life on the order of minutes), relevant to route of administration.
- 5
PubMed (animal studies)Selank effects on serotonin/monoamine metabolism and BDNF expression in rodents (preclinical mechanism data).
- 6
Regulatory statusSelank (Russian trade name) is a registered intranasal anxiolytic in Russia/Ukraine only; it is not approved in the US, EU or UK.
- 7
Peptide Storage GuideBest practices for storing lyophilized peptides (temperature, humidity and light protection).
- 8
Bacteriostatic Water GuidanceBacteriostatic water for injection: multi-dose vial stability and handling.
- 9
NCBI BookshelfBest practices for subcutaneous injection: aseptic technique and site rotation.
- 10
Centers for Disease Control and Prevention (CDC)Subcutaneous injection technique: angle, site and no-aspiration guidance.
- 11
Subcutaneous Injection Technique (Patient Education)How to administer a subcutaneous injection: clinical technique guidelines.
Selank — frequently asked questions
How do I reconstitute a 5 mg vial of Selank?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to Selank?
There is no single correct amount — more water simply spreads the same 5 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store Selank after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 5 mg vial of Selank provide?
Divide the vial strength of 5 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is Selank approved for human use?
No. Selank is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
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