Vilon (20mg Vial) Dosage Protocol
Khavinson immune/thymus dipeptide bioregulator (KE) — research-only; not approved, benefits unproven.
Mix & measure Vilon · 20 mg
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers.
Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →
Quickstart Highlights
Vilon is a synthetic immunoregulatory dipeptide (Lys-Glu, “KE”), modeled from thymus-gland peptide extracts and counted among the Khavinson “cytogen” bioregulators[1][2]. This educational page outlines a pulsed subcutaneous approach (5 consecutive days per 4-week cycle) with a dilution chosen so doses land on easy-to-read insulin-syringe marks. It is an unapproved research chemical, not a medicine; its proposed immune/epigenetic effects are preclinical and unproven in humans — presented for research and educational use only.
Add 3.0 mL bacteriostatic water to one 20 mg vial → ~6.67 mg/mL (6,667 mcg/mL), a practical dilution for clear insulin-syringe measurements.
67–670 mcg once daily for 5 consecutive days per 4-week cycle, repeated monthly for 2–4 cycles.
At ~6.67 mg/mL, 1 unit ≈ 66.7 mcg; doses run from 1 unit (67 mcg) up to 10 units (667 mcg) on a U-100 syringe.
Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and use within ~1 week, or aliquot and freeze.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
Dosing & Reconstitution Guide
A single practical dilution with accurate once-daily dosing, step by step
Pulsed 5-Day Cycle Approach (3 mL = ~6.67 mg/mL)
Reconstitute: Add 3.0 mL bacteriostatic water to one 20 mg vial → final concentration ~6.67 mg/mL (6,667 mcg/mL).
Typical dose: 67–670 mcg once daily for 5 consecutive days per 4-week cycle, repeated monthly.
Easy measuring: At ~6.67 mg/mL, 1 unit ≈ 66.7 mcg on a U-100 syringe. Doses fall between 1 and 10 units (0.01–0.10 mL); 30- or 50-unit syringes improve readability at low volumes.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and use within ~1 week or aliquot and freeze.
Frequency: one subcutaneous injection once daily for 5 consecutive days, then rest for the remainder of the 4-week cycle; repeat monthly for 2–4 cycles (an 8–16 week course). This pulsed pattern is adapted from related short-peptide regimens[3][4]. These figures come from reference protocols, not from approved human dosing.
Reconstitution Steps
Draw 3.0 mL of bacteriostatic water into a sterile syringe.
Release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.
The 3.0 mL dilution keeps doses readable on a U-100 syringe (1–10 units per injection); 30- or 50-unit syringes help at the low end. Use the reconstituted solution within about a week, or split it into frozen aliquots; avoid repeated freeze–thaw, which can denature the peptide.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
Quantities below assume an 8–16 week pulsed course — 5 injection days per 4-week cycle (2–4 cycles).
A 20 mg vial covers the full course at these low pulsed doses, so a single vial is typically enough for 8–16 weeks.
- 8 weeks (2 cycles): ~1 vial
- 12 weeks (3 cycles): ~1 vial
- 16 weeks (4 cycles): ~1 vial
- Per cycle: 5 syringes (1/day × 5 days)
- 8 weeks (2 cycles): ~10 syringes
- 16 weeks (4 cycles): ~20 syringes
Use 3.0 mL per 20 mg vial for reconstitution.
- 8–16 weeks (1 vial): 3 mL → 1 × 10 mL bottle
One for the vial stopper + one for the injection site each day.
- Per cycle: 10 swabs (2/day × 5 days)
- 8–16 weeks: ~20–40 swabs → 1 box
Protocol Overview
A concise summary of the once-daily regimen, drawn from commonly cited reference protocols.
- ▪Goal: Explore immune/thymus-related markers reported for the Lys-Glu dipeptide in preclinical work — effects observed in cell culture and animals, not established in humans[1][3].
- ▪Schedule: Once-daily subcutaneous injections for 5 consecutive days per 4-week cycle; repeat for 2–4 cycles (8–16 weeks).
- ▪Dose Range: 67–667 mcg daily, titrated upward across Cycle 1 (1–5 units) and held at 5–10 units in later cycles.
- ▪Reconstitution: 3.0 mL bacteriostatic water per 20 mg vial gives ~6.67 mg/mL (1 unit ≈ 66.7 mcg).
- ▪Storage: Keep the dry vial frozen at −20 °C (−4 °F); once mixed, refrigerate at 2–8 °C and use within ~1 week or aliquot and freeze.
Dosing Protocol
A suggested daily titration approach based on common reference doses.
- ▪Start: Cycle 1, Day 1 begins at 67 mcg (1 unit), increasing by ~67 mcg (1 unit) each day.
- ▪Titrate: Step up daily through Day 5 of Cycle 1 (67 → 333 mcg; 1 → 5 units).
- ▪Target: From Cycle 2 onward, run 333–667 mcg (5–10 units) daily across the 5 injection days.
- ▪Cycle Length: 5 days on, ~23 days off (a 4-week cycle); run 2–4 cycles (8–16 weeks).
- ▪Timing: Inject at a consistent time on each dosing day and rotate injection sites systematically.
Storage Instructions
Correct storage is what preserves the peptide’s stability and activity.
- ▪Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions and limit moisture exposure[7].
- ▪Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F) and use within about 1 week; for longer storage, aliquot and freeze, avoiding repeated freeze–thaw, which can denature peptides[8].
- ▪Handling: Let frozen vials warm to room temperature before opening so condensation won’t form, and keep the solution clear of heat and direct light.
- ▪Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.
Important Notes
Practical points that keep daily administration safe and consistent.
- ▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
- ▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[9].
- ▪Slow injection: Push the plunger slowly and pause a few seconds before withdrawing the needle to prevent backflow.
- ▪Recordkeeping: Log the daily dose, injection site and any observations to keep the protocol consistent.
- ▪Regulatory note: Vilon is not FDA- or EMA-approved for human use and is research-only; as an unapproved immunomodulator it falls under WADA’s S0/S2 catch-all categories for sport[10].
How This Works
Vilon is a synthetic dipeptide — lysine and glutamic acid (Lys-Glu, the “KE” peptide) — designed by the St. Petersburg (Khavinson) group as the immune/thymus member of their “cytogen” family of short bioregulators, modeled from thymus-gland peptide extracts[1][2].
The proposed mechanism is epigenetic and immunoregulatory: in cell-culture and animal work the dipeptide is reported to alter chromatin packing (“deheterochromatinization”), modulate NF-κB-pathway signalling, and influence cytokine genes such as interleukin-2 and CD5+ lymphocyte populations[3][4]. These are preclinical, cell-level observations, not demonstrated clinical effects.
Most of the evidence comes from the originating Russian group over roughly four decades, with sparse independent Western replication. Reported human data are preclinical or come from uncontrolled, non-blinded observation; there are no registered Western randomized controlled trials[5].
Important caveat: claims of anti-aging, immune-rejuvenation, or “organ regeneration” are not established. The pulsed 5-day monthly schedule on this page is adapted by analogy from related short-peptide bioregulator protocols, not from approved human dosing.
Vilon is not FDA- or EMA-approved. It is an unapproved research chemical presented here for research and educational purposes only.
Lifestyle Factors
Habits that may support immune health alongside the protocol.
- ▪Nutrition: Favor a nutrient-dense diet with adequate protein and antioxidants to support general immune function.
- ▪Activity & rest: Pair regular moderate exercise with genuine recovery time to reinforce immune and metabolic health.
- ▪Sleep: Aim for 7–9 hours to support the body’s natural immune regulation.
- ▪Stress: Manage stress with evidence-based practices, since it influences overall immune health.
Potential Benefits & Side Effects
What preclinical and cell-culture literature describe; human evidence is limited and unproven, and individual results vary.
Potential Benefits
- ▪Immune markers (preclinical): Cell-culture work reports increased CD5+ lymphocytes in thymus cultures and upregulated IL-2 gene expression in splenocytes[1][2].
- ▪Cytokine modulation (preclinical): In experimental renal-stress models the dipeptide has been linked to changes in TGF-β1 and vascular permeability[3].
- ▪Animal models: Early rodent studies suggested possible tumor-growth inhibition and lifespan effects; these are not replicated in controlled human trials[6].
- ▪Note on humans: These benefits are not established in humans — evidence is dominated by the originating Russian group, with no registered Western RCTs[5].
Common Side Effects
- ▪Injection-site reactions: Mild redness, tenderness or soreness can occur; rotating sites helps.
- ▪Unknown long-term profile: Human safety data is limited, so caution and monitoring are advised.
- ▪Sport restriction: As an unapproved immunomodulator, Vilon falls under WADA’s S0/S2 catch-all categories for athletes.
Injection Technique
General subcutaneous technique, following established clinical best-practice guidance.
Pre-Injection Preparation
- ▪Wash your hands well with soap and water.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully.
- ▪Draw the intended dose, then check for air bubbles and push any out.
Injection Procedure
- ▪Pinch a skinfold at the chosen site between thumb and forefinger.
- ▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin).
- ▪Skip aspiration for subcutaneous shots — it isn’t needed.
- ▪Press the plunger slowly and steadily until it’s fully down.
- ▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
Post-Injection Care
- ▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away.
- ▪Rotate the injection site each day to prevent irritation and lipohypertrophy[9].
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
Vilon is a niche research peptide and is not sold by our usual partner. For general high-purity research peptides and supplies, we point researchers to Prime Lab Peptides.
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
Sevostianova NN et al. (2013), Bull Exp Biol MedImmunomodulating effects of Vilon and its analogue in cultures of human and animal thymus cells (CD5+ lymphocytes).
- 2
Kazakova TB et al. (2002)In vitro effect of short peptides on interleukin-2 (IL-2) gene expression in splenocytes.
- 3
Gavrisheva NA et al. (2005), Bull Exp Biol MedEffect of the Vilon peptide on TGF-β1 and vascular permeability in chronic renal failure (animal model).
- 4
Kniaz’kin IA et al. (2002), Adv GerontolEffect of the peptide Vilon on markers of immune aging (originating-group research).
- 5
Araj SK et al. (2025), Int J Mol SciOverview of Epitalon, a related short peptide bioregulator — cited for the analogous pulsed-cycle regimen, not Vilon-specific human efficacy.
- 6
Chernyak EY et al. (2000), Dokl Biol SciSynthetic dipeptide Vilon and tumor growth / lifespan effects in mice (uncontrolled animal data).
- 7
Merck (MilliporeSigma) / GenScriptHandling and storage guidelines for lyophilized peptides (temperature, humidity, light).
- 8
Bacteriostatic Water GuidanceBacteriostatic water for injection: multi-dose vial stability and handling.
- 9
MedlinePlus (NIH)Subcutaneous (SQ) injection: patient information, technique and site rotation.
- 10
WADA Prohibited ListUnapproved immunomodulators fall under the S0/S2 catch-all categories in competitive sport.
- 14
Centers for Disease Control and Prevention (CDC)Subcutaneous injection technique: angle, site and no-aspiration guidance.
- 15
NCBI BookshelfBest practices for subcutaneous injection: aseptic technique and administration.
How to reconstitute Vilon
- 1Wipe the vial stopper and your bacteriostatic-water vial with an alcohol swab.
- 2Draw your chosen amount of bacteriostatic water and inject it slowly down the inner wall of the Vilon vial.
- 3Let it rest, then gently swirl until fully dissolved — do not shake.
- 4Refrigerate the reconstituted vial and draw each dose with a U-100 insulin syringe.
Vilon — frequently asked questions
How do I reconstitute a 20 mg vial of Vilon?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to Vilon?
There is no single correct amount — more water simply spreads the same 20 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store Vilon after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 20 mg vial of Vilon provide?
Divide the vial strength of 20 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is Vilon approved for human use?
No. Vilon is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
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