HMG (75IU Vial) Dosage Protocol
Menotropins (FSH + LH) — FDA-approved for female fertility; the male-fertility protocol here is off-label.
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers. Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →Mix & measure HMG · 75 IU
Dosing & Reconstitution Guide
Off-label male-fertility reconstitution and thrice-weekly dosing, step by step
Male Fertility Protocol (3.0 mL = 25 IU/mL)
Reconstitute: Add 3.0 mL bacteriostatic water to one 75 IU vial → concentration 25 IU/mL.
Typical male protocol: 75 IU three times weekly (e.g. Monday, Wednesday, Friday) for 12 weeks, optionally extended to 16, usually combined with hCG therapy[3][5].
Unit math: on a U-100 syringe, 1 unit = 0.01 mL = 0.25 IU at this dilution. So 75 IU = 3.0 mL = 300 units, 25 IU = 1.0 mL = 100 units.
Storage: Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F); after reconstitution use promptly or refrigerate and use within a few days.
Why HMG draws research interest
These are the directions researchers and the peptide community most often explore HMG for — so you know you’re in the right place. They describe what is being studied, not proven benefits, approved uses, or promised results.
Male fertility
Investigated off-label for supporting sperm production, since HMG supplies both FSH and LH to the testes; not FDA-approved for this use.
Female fertility & IVF
FDA-approved as menotropins for ovulation induction and ovarian stimulation in assisted-reproduction protocols such as IVF.
Testicular function support
Explored in research on maintaining testicular activity alongside testosterone therapy, where natural LH signaling is suppressed.
FSH + LH signaling
Studied as a dual gonadotropin source that delivers both follicle-stimulating and luteinizing hormone activity in one preparation.
Evidence ranges from early laboratory work to clinical trials depending on the use — the sections below cover the actual data and sources.
Quickstart Highlights
HMG (human menopausal gonadotropin / menotropins) is a urinary-derived gonadotropin preparation carrying roughly equal FSH and LH activity — FSH drives follicular growth in women and Sertoli-cell-supported spermatogenesis in men, while LH activity drives steroidogenesis (Leydig-cell testosterone in men)[1][2]. Menotropins (brand Menopur) is FDA-approved — but for controlled ovarian stimulation and ovulation induction in women. The thrice-weekly, hCG-combination male-fertility protocol outlined here is off-label clinical practice requiring physician oversight, and the research-grade vial described is not the licensed Menopur product. Presented for educational use only.
Add 3.0 mL bacteriostatic water to one 75 IU vial → 25 IU/mL for calculation convenience.
75 IU three times weekly (e.g. Mon/Wed/Fri) for 12 weeks (optionally 16), usually combined with hCG therapy.
At 25 IU/mL, a full 75 IU dose = 3.0 mL = 300 units — the entire vial, which exceeds a 1 mL insulin syringe. See the volume note below.
Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F); after reconstitution, use promptly or refrigerate and use within a few days.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
Important volume note: Each full 75 IU dose at 25 IU/mL is 3.0 mL = 300 units = the entire reconstituted vial, which exceeds standard 1 mL insulin-syringe capacity. Options: (1) use a 3 mL syringe for a single injection; (2) split the dose into three ~1 mL (25 IU) injections at different sites in one session; or (3) reconstitute in a smaller volume — e.g. 1.0 mL bacteriostatic water gives 75 IU/mL, so 1.0 mL = 100 units delivers the full dose[7].
Frequency: three subcutaneous injections per week, typically alongside hCG (2–3× weekly) to support testosterone and spermatogenesis[5][6]. These figures reflect off-label reference protocols, not approved male dosing.
Reconstitution Steps
Draw 3.0 mL bacteriostatic water with a sterile 3 mL syringe.
Inject slowly down the vial wall to avoid foaming.
Gently swirl or roll until fully dissolved — do not shake.
Label with date and time; refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
The 3.0 mL dilution gives a tidy 25 IU/mL for calculation convenience, but means a full dose fills the whole vial. If a smaller injection volume is preferred, a 1.0 mL reconstitution (75 IU/mL) lets the full 75 IU dose fit in a single 1 mL syringe (100 units). Use reconstituted solution promptly.
Supplies Needed
Quantities below assume a 12–16 week male-fertility course with thrice-weekly administration.
One full 75 IU dose uses an entire vial, so plan three vials per week.
- 12 weeks (3/week): ~36 vials
- 16 weeks (3/week): ~48 vials
A 3 mL syringe holds the full 3.0 mL dose. If using 1 mL insulin syringes for split dosing, multiply counts by 3.
- Per week: 3 syringes
- 12 weeks: ~36 syringes
- 16 weeks: ~48 syringes
Use 3.0 mL per 75 IU vial for reconstitution.
- 12 weeks (36 vials): ~108 mL → ~11 bottles
- 16 weeks (48 vials): ~144 mL → ~15 bottles
One for the vial stopper + one for each injection site.
- Per injection day: 2 swabs
- 12 weeks (3/week): ~72 swabs → 1 box
- 16 weeks (3/week): ~96 swabs → 1 box
Protocol Overview
A concise summary of the off-label thrice-weekly male-fertility regimen, drawn from clinical reference protocols.
- ▪Goal: Support spermatogenesis in men with hypogonadotropic hypogonadism or secondary infertility by supplying FSH activity alongside hCG — an off-label use, not an established benefit[3][5].
- ▪Schedule: Subcutaneous injections three times weekly for a minimum of 12 weeks, extendable to 16 weeks based on response[7].
- ▪Dose: 75 IU per injection, typically combined with hCG therapy[5][6].
- ▪Reconstitution: 3.0 mL bacteriostatic water per 75 IU vial gives 25 IU/mL for calculation convenience.
- ▪Storage: Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F); reconstituted solution used promptly or within a few days when refrigerated.
Dosing Protocol
Standard off-label male-fertility support approach.
- ▪Dose: 75 IU three times per week (e.g., Monday, Wednesday, Friday).
- ▪Combination therapy: Usually administered alongside hCG (2–3 times weekly) to support testosterone and spermatogenesis[5][6].
- ▪Cycle length: Minimum 12 weeks; may extend to 16 weeks based on response[7].
- ▪Route: Subcutaneous injection into fatty tissue (abdomen, thigh, or upper arm).
- ▪Timing: Maintain consistent injection days; rotate sites with each injection.
Storage Instructions
Proper storage maintains HMG stability and potency.
- ▪Lyophilized (unopened): Store at 2–8 °C (35.6–46.4 °F) away from light; stable at controlled room temperature up to 25 °C (77 °F)[8].
- ▪Reconstituted: Refrigerate at 2–8 °C; for best potency use promptly (official guidance recommends immediate use with plain diluent)[8].
- ▪With bacteriostatic water: Reconstituted solution may be refrigerated and used within a few days.
- ▪Light & disposal: Protect from light at all times; discard any unused solution if not used within the recommended timeframe.
Important Notes
Practical considerations for safe and consistent administration.
- ▪Combination therapy: Male protocols typically require concurrent hCG administration; HMG alone is not used to raise testosterone or boost fertility as an established benefit[5][6].
- ▪Sterile technique: Use a new sterile syringe for each injection and dispose in a puncture-proof sharps container[9].
- ▪Site rotation: Rotate sites (abdomen, thighs, upper arms) at least 1 inch apart to prevent lipohypertrophy or irritation.
- ▪Volume: The 3.0 mL injection volume may require a 3 mL syringe, splitting into smaller injections, or a smaller reconstitution volume.
- ▪Monitoring: Hormone therapy can disrupt the HPG axis — monitor semen parameters and hormone levels throughout under physician oversight. Clinical response typically takes at least 12 weeks; spermatogenesis may take 3–6 months to fully develop[7].
- ▪Regulatory note: Menotropins is FDA-approved for ovulation induction in women; male fertility use is off-label. Gonadotropins (S2.3) — and the co-therapy hCG — are WADA-prohibited in male athletes[10].
How This Works
HMG (human menopausal gonadotropin / menotropins) is a urinary-derived gonadotropin carrying roughly equal FSH and LH activity (standard preparations provide about 75 IU FSH + 75 IU LH per vial)[1][2].
FSH activity drives follicular growth in women and supports Sertoli-cell-mediated spermatogenesis in men; LH activity drives steroidogenesis — Leydig-cell testosterone production in men[1]. In men with hypogonadotropic hypogonadism, FSH is essential for spermatogenesis; when hCG alone fails to induce adequate sperm production, adding HMG supplies the FSH activity needed to support testicular function[3][5].
Clinical fertility literature reports that HMG combined with hCG can improve sperm parameters (motility, morphology, concentration) and pregnancy rates in treated couples[7]. These outcomes come from supervised clinical use, not from self-administered research-grade product.
Approval status: menotropins (brand Menopur) is FDA-approved — but for controlled ovarian stimulation and ovulation induction in women undergoing assisted reproduction. The thrice-weekly, hCG-combination male-fertility protocol on this page is off-label clinical practice requiring physician oversight and bloodwork, and the research-grade vial described here is not the licensed Menopur product. We do not claim it raises testosterone or boosts fertility as an established benefit in men.
Gonadotropin therapy can disrupt the hypothalamic-pituitary-gonadal (HPG) axis and is not appropriate for casual self-administration. Presented for educational purposes only.
Lifestyle Factors
Complementary habits that may support fertility outcomes alongside supervised therapy.
- ▪Nutrition & weight: Maintain a healthy body weight and a balanced diet rich in antioxidants (zinc, selenium, vitamins C and E).
- ▪Heat exposure: Avoid excessive heat to the testes (hot tubs, saunas, tight clothing).
- ▪Substances: Limit alcohol and avoid tobacco and recreational drugs.
- ▪Sleep & stress: Prioritize adequate sleep and manage stress; moderate exercise supports hormonal balance (avoid excessive endurance training).
- ▪Supplements: Consider coenzyme Q10, L-carnitine or other evidence-based supplements after consultation with a clinician.
Potential Benefits & Side Effects
Observations from clinical fertility literature; outcomes depend on supervised use and individual response.
Potential Benefits
- ▪Spermatogenesis support (off-label): May stimulate sperm production in men with hypogonadotropic hypogonadism or secondary infertility when combined with hCG[3].
- ▪Sperm parameters: Literature reports improved motility, morphology and concentration with HMG + hCG therapy[7].
- ▪Pregnancy rates: Increased pregnancy rates reported in couples undergoing supervised fertility treatment[7].
- ▪Note on men: These benefits are off-label and physician-supervised — HMG is FDA-approved for ovulation induction in women, not for raising testosterone or boosting male fertility as an established indication.
Common Side Effects
- ▪Injection-site reactions: Redness, swelling or mild pain; rotating sites helps.
- ▪Systemic effects: Headache, fatigue or mood changes (uncommon); gynecomastia from hormonal stimulation.
- ▪Overstimulation & allergy: Overstimulation effects if dosing is excessive (rare in men with proper monitoring); allergic reactions are rare — discontinue if hypersensitivity occurs.
- ▪Sport restriction: Gonadotropins (and co-therapy hCG) are WADA-prohibited in male athletes.
Injection Technique
General subcutaneous technique, following established clinical best-practice guidance[9].
Pre-Injection Preparation
- ▪Wash your hands thoroughly with soap and water and gather supplies on a clean surface.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Draw the prescribed dose — a 3 mL syringe for the full 75 IU (3.0 mL) dose, or split across three 1 mL (25 IU) injections.
- ▪Select a site (lower abdomen at least 2 inches from the navel, outer thigh, or upper arm), clean it with a fresh alcohol swab and let it dry fully.
Injection Procedure
- ▪Pinch about an inch of skin to create a fold of subcutaneous tissue.
- ▪Insert the needle at a 90-degree angle (or 45 degrees if there is little subcutaneous fat).
- ▪Release the pinch and inject slowly over several seconds.
- ▪Withdraw the needle smoothly and apply gentle pressure with sterile gauze — do not rub the site.
- ▪Split-dose: If using 1 mL insulin syringes, divide the 3.0 mL total into three 1 mL injections (25 IU each) at different sites in the same session.
Post-Injection Care
- ▪Dispose of the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away, protected from light.
- ▪Rotate the injection site each session, keeping at least 1 inch from previous sites to prevent irritation and lipohypertrophy.
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
For high-purity research peptides, we point researchers to Prime Lab Peptides.
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
FSH + LH composition (menotropins)HMG is a urinary-derived gonadotropin with roughly equal FSH and LH activity; standard vials provide ~75 IU FSH + 75 IU LH.
- 2
Menopur (Menotropins) Product MonographFDA-approved human menopausal gonadotropin for subcutaneous administration in controlled ovarian stimulation; ~75 IU FSH + 75 IU LH per vial.
- 3
Male hypogonadotropic hypogonadism (PubMed)Gonadotropin therapy for spermatogenesis in hypogonadotropic hypogonadism; FSH (HMG) added when hCG alone is insufficient.
- 4
ASRM Practice Committee – Gonadotropins for Ovulation InductionClinical guidance on gonadotropin therapy; low-dose protocols (37.5-75 IU) with careful monitoring (approved use is in women).
- 5
HMG + hCG male fertility (PubMed)Combination gonadotropin therapy to support testosterone and spermatogenesis in men; off-label clinical practice.
- 6
Combination therapy outcomes (PubMed)HMG combined with hCG and reported effects on sperm motility, morphology and concentration.
- 7
Spermatogenesis timeline (PubMed)Clinical response to gonadotropin therapy typically requires 12+ weeks; spermatogenesis may take 3-6 months to develop.
- 8
Menotropins storage & handlingStorage of lyophilized and reconstituted menotropins: refrigerate at 2-8 C, protect from light, use reconstituted solution promptly.
- 9
NCBI Bookshelf – Subcutaneous injection techniqueBest practices for subcutaneous injection: aseptic technique, angle and site rotation.
- 10
WADA Prohibited ListGonadotropins (S2.3) and the co-therapy hCG are prohibited in male athletes.
- 11
Prime Lab PeptidesHMG (75 IU) research-grade product source – purity specifications and certificates of analysis.
HMG — frequently asked questions
How do I reconstitute a 75 IU vial of HMG?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to HMG?
There is no single correct amount — more water simply spreads the same 75 IU of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store HMG after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 75 IU vial of HMG provide?
Divide the vial strength of 75 IU by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is HMG approved for human use?
No. HMG is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
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