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Gonadorelin (2 mg Vial) Dosage Protocol

Contents

Quickstart Highlights

Gonadorelin is a synthetic gonadotropin-releasing hormone (GnRH) that stimulates pituitary release of LH and FSH[1]. It is used to support gonadal function and has been studied as an alternative to hCG for maintaining testicular function in men on testosterone replacement therapy[2]. This educational protocol presents a once-daily subcutaneous approach using practical dilution for accurate insulin-syringe measurements.

  • Reconstitute: Add 2.0 mL bacteriostatic water → 1.0 mg/mL concentration.
  • Typical daily range: 100–250 mcg once daily (gradual titration).
  • Easy measuring: At 1.0 mg/mL, 1 unit = 0.01 mL = 10 mcg on a U-100 insulin syringe.
  • Storage: Lyophilized: refrigerate at 2–8 °C (36–46 °F) or −20 °C (−4 °F) for long-term; after reconstitution, refrigerate at 2–8 °C (36–46 °F); do not freeze reconstituted solution.
Gonadorelin (2 mg Vial)
📘 Important: Before viewing any protocol, please consult our Prep & Injection Guide for essential preparation and safety instructions.

Dosing & Reconstitution Guide

Educational guide for reconstitution and daily dosing

Standard / Gradual Approach (2 mL = 1.0 mg/mL)

Week Daily Dose (mcg / mg) Units (per injection) (mL)
Weeks 1–2 100 mcg (0.1 mg) 10 units (0.10 mL)
Weeks 3–4 150 mcg (0.15 mg) 15 units (0.15 mL)
Weeks 5–8 200 mcg (0.2 mg) 20 units (0.20 mL)
Weeks 9–12 250 mcg (0.25 mg) 25 units (0.25 mL)

Frequency: Inject once daily subcutaneously. This schedule maintains optimal pulsatile-like LH/FSH release patterns[1]. For administrations at 10 units (0.10 mL), consider using 30-unit or 50-unit insulin syringes for improved readability and precision.

Reconstitution Steps

  1. Draw 2.0 mL bacteriostatic water with a sterile syringe.
  2. Inject slowly down the vial wall; avoid foaming.
  3. Gently swirl/roll until dissolved (do not shake).
  4. Label with reconstitution date and refrigerate at 2–8 °C (36–46 °F), protected from light.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration.

  • Peptide Vials (Gonadorelin, 2 mg each):
    • 8 weeks ≈ 5 vials
    • 12 weeks ≈ 9 vials
    • 16 weeks ≈ 12 vials
  • Insulin Syringes (U-100, 30- or 50-unit recommended):
    • Per week: 7 syringes (1/day)
    • 8 weeks: 56 syringes
    • 12 weeks: 84 syringes
    • 16 weeks: 112 syringes
  • Bacteriostatic Water (10 mL bottles): Use 2.0 mL per vial for reconstitution.
    • 8 weeks (5 vials): 10 mL → 1 × 10 mL bottle
    • 12 weeks (9 vials): 18 mL → 2 × 10 mL bottles
    • 16 weeks (12 vials): 24 mL → 3 × 10 mL bottles
  • Alcohol Swabs: One for the vial stopper + one for the injection site each day.
    • Per week: 14 swabs (2/day)
    • 8 weeks: 112 swabs → recommend 2 × 100-count boxes
    • 12 weeks: 168 swabs → recommend 2 × 100-count boxes
    • 16 weeks: 224 swabs → recommend 3 × 100-count boxes
Peptide Vial
Insulin Syringes
Bacteriostatic Water
Alcohol Pads

Protocol Overview

Concise summary of the once-daily regimen.

  • Goal: Support natural LH/FSH release to maintain gonadal function[1][2].
  • Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).
  • Dose Range: 100–250 mcg daily with gradual titration.
  • Reconstitution: 2.0 mL per 2 mg vial (1.0 mg/mL) for accurate unit measurements.
  • Storage: Lyophilized refrigerated or frozen; reconstituted refrigerated; avoid freeze-thaw cycles.

Dosing Protocol

Suggested daily titration approach.

  • Start: 100 mcg daily for 2 weeks to assess tolerance.
  • Increase: Progress to 150 mcg (Weeks 3–4), then 200 mcg (Weeks 5–8).
  • Target: 200–250 mcg daily as tolerated.
  • Frequency: Once per day (subcutaneous).
  • Cycle Length: 8–12 weeks; optional extension to 16 weeks with medical guidance.
  • Timing: Any consistent time; rotate injection sites daily.

Storage Instructions

Proper storage preserves peptide quality and potency.

  • Lyophilized: Store at 2–8 °C (36–46 °F) or −20 °C (−4 °F) for long-term storage; protect from light and moisture[4][5].
  • Reconstituted: Refrigerate at 2–8 °C (36–46 °F); do not freeze liquid peptide[4].
  • Stability: Use reconstituted vials within 30 days when refrigerated[3][5].
  • Allow vials to reach room temperature before opening to reduce condensation.

Important Notes

Practical considerations for consistency and safety.

  • Use new sterile insulin syringes for each injection; dispose in a sharps container.
  • Rotate injection sites (abdomen at least 2 inches from navel, outer thigh, or outer upper arm) to prevent irritation or lipoatrophy[6][7].
  • Inject slowly; wait a few seconds before withdrawing the needle.
  • Mark the date of reconstitution on each vial and discard after 30 days.
  • Document daily dose and site rotation to maintain consistency.

How This Works

Gonadorelin is a synthetic form of gonadotropin-releasing hormone (GnRH) that stimulates the anterior pituitary to release luteinizing hormone (LH) and follicle-stimulating hormone (FSH)[1]. In men on testosterone replacement therapy, endogenous GnRH/LH signaling is suppressed; gonadorelin administration can help maintain testicular function by restoring this signaling pathway[2]. Clinical studies have shown that pulsatile GnRH therapy is superior to hCG for promoting testicular growth in hypogonadal patients[9]. The peptide works by mimicking the natural pulsatile release pattern of GnRH, though practical daily dosing protocols (100–250 mcg once daily) approximate the physiological total daily exposure achieved with more frequent pulsatile dosing[1][2].

Potential Benefits & Side Effects

Observations from clinical and preclinical literature.

  • Supports natural LH and FSH production to maintain gonadal function during testosterone therapy[2].
  • May help preserve testicular size and function, with evidence of superior efficacy compared to hCG in adolescent hypogonadism[9].
  • Generally well tolerated; occasional mild injection-site reactions (redness, itching) may occur with subcutaneous administration.
  • Excessively high or frequent dosing can lead to pituitary receptor downregulation, reducing effectiveness[1]; adherence to recommended dosing ranges is important.

Lifestyle Factors

Complementary strategies for optimal hormonal health.

  • Maintain a balanced diet with adequate protein, healthy fats, and micronutrients to support hormone production.
  • Engage in regular resistance training and cardiovascular exercise to support metabolic health.
  • Prioritize 7–9 hours of quality sleep per night for optimal hormonal balance.
  • Manage stress through mindfulness, adequate rest, and recovery practices.
  • Avoid excessive alcohol consumption and minimize exposure to endocrine disruptors.

Injection Technique

General subcutaneous guidance from clinical best-practice resources[6][7][8].

  • Clean the vial stopper and injection site with alcohol; allow to air-dry for ~30 seconds[7].
  • Choose an appropriate site (lower abdomen at least 2 inches from navel, outer thigh, or outer upper arm)[6].
  • Pinch a skinfold between thumb and forefinger; insert the needle at 45–90° depending on the amount of subcutaneous fat[6][7].
  • Do not aspirate for subcutaneous injections – aspiration is not required and can be skipped[7].
  • Inject slowly and steadily; withdraw the needle at the same angle as insertion.
  • Apply gentle pressure with gauze; do not rub or massage the injection site[8].
  • Rotate sites systematically to avoid lipohypertrophy and tissue irritation[6].
  • Dispose of used syringes immediately in a proper sharps container without recapping[8].

Recommended Source

We recommend Prime Lab Peptides for high-purity Gonadorelin (2 mg).

 

Why Prime Lab Peptides?

Important Note

This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment. Gonadorelin is for research use only and is not approved for human consumption. Always consult with a qualified healthcare provider before beginning any peptide protocol, especially if you have pre-existing medical conditions or are taking medications. Individual responses to peptides may vary, and proper medical supervision is essential for safety and efficacy.

References


  • Drugs.com (AHFS/Micromedex)
    — Gonadorelin injectable usage and dosing information (clinical reference)
    View Source

  • Medicina (MDPI) 2024
    — Management of male fertility in hypogonadal patients on testosterone replacement therapy
    View Source

  • Int J Fertil 1990 (PubMed)
    — Stability and purity profile of gonadorelin acetate (high-purity GnRH)
    View Source

  • Health Canada
    — Lutrepulse (gonadorelin acetate) product monograph and storage guidance
    View Source

  • Rosemont Specialty Pharmacy 2021
    — Fertility medications and storage information (gonadorelin and related peptides)
    View Source

  • Immunize.org (IAC/CDC)
    — How to administer intramuscular and subcutaneous vaccine injections (technique guide)
    View Source

  • NCBI Bookshelf
    — Chapter 18: Administration of parenteral medications (nursing skills reference)
    View Source

  • Elsevier Clinical Skills
    — Medication administration: subcutaneous injections (home health care guide)
    View Source

  • J. Clin. Endocrinol. Metab. 2015
    — Pulsatile GnRH is superior to hCG in therapeutic efficacy in adolescent boys with hypogonadotropic hypogonadism
    View Source