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CJC-1295 no DAC + Ipamorelin (GROW-H 10mg) Dosage Protocol

GHRH analog (CJC-1295 no DAC) + ghrelin GH secretagogue (Ipamorelin) in one blend — research-only; not FDA-approved.

Peptide Stacks Updated November 25, 2025 1 min read Research information only
CJC-1295 no DAC + Ipamorelin (GROW-H 10mg) Dosage Protocol
Why researchers study it

Why CJC-1295 no DAC + Ipamorelin draws research interest

These are the directions researchers and the peptide community most often explore CJC-1295 no DAC + Ipamorelin for — so you know you’re in the right place. They describe what is being studied, not proven benefits, approved uses, or promised results.

GH & IGF-1 support

Investigated in research on stimulating pulsatile growth hormone release and downstream IGF-1; not approved for any therapeutic use.

Fat metabolism & lipolysis

Explored in preclinical research on how growth hormone secretagogues influence fat breakdown and body composition.

Recovery & tissue repair

Studied for growth hormone's role in connective tissue and muscle repair; investigational only, with no approved use.

Sleep quality

Investigated in early research on links between growth hormone secretagogues and slow-wave (deep) sleep.

Research context, not medical advice. Nothing here says CJC-1295 no DAC + Ipamorelin treats, cures, or improves any condition. Evidence ranges from early laboratory work to clinical trials depending on the use — the sections below cover the actual data and sources.

Reconstitution calculator

Mix & measure CJC-1295 no DAC + Ipamorelin · 10 mg

Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers.

Concentrationmg/mL
Draw volumemL
On the syringeunits
Doses / vial 

Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →

Quick answerIn the research literature the 10 mg CJC-1295 no DAC + Ipamorelin vial is typically reconstituted with 3 mL of bacteriostatic water, and documented research protocols reference 300 mcg per dose drawn from the resulting solution. These figures reflect published research conventions rather than clinical guidance, and are provided strictly for research use only.
01 · At a glance

Quickstart Highlights

GROW-H is a single combined 10 mg blend vial pairing CJC-1295 no DAC (modified GRF 1-29, a short-acting GHRH analog) with Ipamorelin (a selective ghrelin/GHS-R agonist). Together a GHRH analog and a GHRP are intended to amplify the body’s own pulsatile growth-hormone release. Both are unapproved research chemicals — neither is FDA-approved — and the long-term body-composition, recovery and sleep benefits of this blend are not validated by controlled human trials. Presented for research and educational use only.

Reconstitute

One 10 mg combined vial. Add 3.0 mL bacteriostatic water → 3,333 mcg/mL (Standard), or 2.0 mL5,000 mcg/mL (Advanced).

Daily range

300–1,200 mcg total blend once daily, on a 5-days-on / 2-days-off schedule, titrated upward across the cycle.

Easy measuring

Doses are total blend. At 3,333 mcg/mL, 1 unit ≈ 33.3 mcg; at 5,000 mcg/mL, 1 unit = 50 mcg on a U-100 syringe.

Storage

Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the solution.

Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.

02 · Dosing & reconstitution

Dosing & Reconstitution Guide

One combined 10 mg vial — two dilution options for once-daily total-blend dosing

All doses below are stated as total blend (combined CJC-1295 no DAC + Ipamorelin). For a 10 mg blend the split is typically ~5 mg of each, though this is not labeled on the page; because both peptides share one vial, you cannot draw a single component separately. Run once daily on a 5-days-on / 2-days-off schedule to limit receptor desensitization.

Standard / Gradual Approach (3 mL = 3,333 mcg/mL)

Reconstitute: Add 3.0 mL bacteriostatic water to the 10 mg combined vial3,333 mcg/mL.

Typical daily range: 300–700 mcg total blend once daily, raised gradually across the cycle.

Easy measuring: At 3,333 mcg/mL, 1 unit ≈ 33.3 mcg on a U-100 syringe (units = mcg ÷ 33.33).

Phase / Week(s) Total Blend Dose & Frequency Volume (U-100 units / mL)
Weeks 1–4 300 mcg (1× daily) ~9 units (0.09 mL)
Weeks 5–8 500 mcg (1× daily) ~15 units (0.15 mL)
Weeks 9–12 600 mcg (1× daily) ~18 units (0.18 mL)
Weeks 13–16 (optional) 700 mcg (1× daily) ~21 units (0.21 mL)

Advanced / Aggressive Approach (2 mL = 5,000 mcg/mL)

Reconstitute: Add 2.0 mL bacteriostatic water to the 10 mg combined vial5,000 mcg/mL. The smaller volume keeps higher doses comfortably above 10 units.

Typical daily range: 600–1,200 mcg total blend once daily, for researchers targeting higher doses.

Easy measuring: At 5,000 mcg/mL, 1 unit = 50 mcg on a U-100 syringe (units = mcg ÷ 50).

Phase / Week(s) Total Blend Dose & Frequency Volume (U-100 units / mL)
Weeks 1–4 600 mcg (1× daily) ~12 units (0.12 mL)
Weeks 5–8 800 mcg (1× daily) ~16 units (0.16 mL)
Weeks 9–12 1,000 mcg (1× daily) ~20 units (0.20 mL)
Weeks 13–16 (optional) 1,200 mcg (1× daily) ~24 units (0.24 mL)

Frequency: one subcutaneous injection each day on a 5-on / 2-off pattern, titrating up as tolerated. Pairing a GHRH analog (CJC-1295 no DAC) with a GHRP (Ipamorelin) is intended to amplify natural pulsatile GH release; the acute synergy is pharmacologically reasonable, but these figures come from common supplier reference protocols, not from approved human dosing.

Reconstitution Steps

1

Draw 3.0 mL (Standard) or 2.0 mL (Advanced) of bacteriostatic water into a sterile syringe.

2

Release it slowly down the vial’s inner wall to limit foaming.

3

Swirl or roll gently until fully dissolved — don’t shake.

4

Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.

Note

The 3.0 mL dilution favors lower doses with simple math (1u ≈ 33.3 mcg); the 2.0 mL dilution suits higher doses while keeping units readable (1u = 50 mcg). Avoid freezing the reconstituted solution, since freeze–thaw can denature the peptides.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

03 · What you’ll need

Supplies Needed

Quantities below assume an 8–16 week course of once-daily injections (5-on / 2-off) with gradual titration.

Blend Vials (GROW-H, 10 mg combined)

One combined 10 mg vial (CJC-1295 no DAC + Ipamorelin). At ~5 mg of blend used per week, a 10 mg vial covers roughly two weeks.

  • 8 weeks: ~4 vials
  • 12 weeks: ~6 vials
  • 16 weeks: ~8 vials
Insulin Syringes (U-100, 1 mL)
  • Per injection: 1 syringe
  • 8 weeks (5-on/2-off): ~40 syringes
  • 16 weeks: ~80 syringes
Bacteriostatic Water (10 mL bottles)

Use 2.0–3.0 mL per 10 mg vial for reconstitution.

  • 8 weeks (4 vials): ~12 mL2 bottles
  • 16 weeks (8 vials): ~24 mL3 bottles
Alcohol Swabs

One for the vial stopper + one for the injection site each day.

  • Per injection: 2 swabs
  • 8 weeks (5-on/2-off): ~80 swabs1 box
GROW-H 10 mg Blend Vial
Blend Vial

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Insulin Syringes
Insulin Syringes

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Bacteriostatic Water
Bacteriostatic Water

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Alcohol Pads
Alcohol Pads

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Protocol Overview

A concise summary of the once-daily regimen, drawn from common supplier reference protocols.

  • Goal: Amplify the body’s own pulsatile GH release by pairing a GHRH analog (CJC-1295 no DAC) with a GHRP (Ipamorelin) — an acute, pharmacologically reasonable synergy; longer-term recovery and body-composition benefits are not established in controlled human trials.
  • Schedule: Once-daily subcutaneous injections, typically 5 days on, 2 days off, for 8–16 weeks.
  • Dose Range: 300–600 mcg total blend daily for most researchers, with advanced use reaching 1,000–1,200 mcg.
  • Reconstitution: 3.0 mL → 3,333 mcg/mL (Standard) or 2.0 mL → 5,000 mcg/mL (Advanced) per 10 mg vial.
  • Storage: Keep the dry vial frozen at −20 °C; once mixed, refrigerate at 2–8 °C and do not freeze the solution.

Dosing Protocol

A suggested daily titration approach based on common reference doses (all figures total blend).

  • Start: Begin at ~300 mcg once daily to gauge tolerability.
  • Titrate: Increase gradually every few weeks as tolerated.
  • Target: Reach about 600 mcg daily (Standard) or up to 1,000–1,200 mcg (Advanced) over the cycle.
  • Cycle Length: Typically 8–16 weeks, followed by a rest period to maintain receptor sensitivity.
  • Timing: Best on an empty stomach — morning or before bed — injected at a consistent time, rotating sites.

Storage Instructions

Correct storage is what preserves the peptides’ stability and activity.

  • Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions.
  • Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F) and use within about 28–30 days; do not freeze the mixed solution.
  • Handling: Let frozen vials warm to room temperature before opening, and keep the solution clear of heat and direct light.
  • Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.
04 · Good to know

Important Notes

Practical points that keep daily administration safe and consistent.

  • Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a sharps container afterward.
  • One combined vial: CJC-1295 no DAC and Ipamorelin share a single vial, so each dose draws both at once — you cannot measure or inject one component separately.
  • Empty stomach: Inject on an empty stomach and wait ~30 minutes before eating; site-rotate across abdomen, thighs and upper arms.
  • Monitoring: Track dose, site and observations weekly; discontinue if serious reactions occur.
  • Regulatory note: Both peptides are research chemicals, not FDA-approved (removed from 503A compounding Category 2 in Sept 2024; status in flux), and both are WADA-prohibited (class S2) for athletes.
05 · How it works

How This Works

CJC-1295 no DAC (modified GRF 1-29) is a short-acting GHRH analog. It mimics growth-hormone-releasing hormone at the pituitary for a brief window (~30 minutes), nudging out a pulse of GH without driving a sustained, tonic rise in IGF-1.

Ipamorelin is a selective ghrelin / GHS-R agonist (a growth-hormone-releasing peptide, GHRP). It triggers a clean GH pulse with minimal effect on cortisol or prolactin.

Pairing a GHRH analog with a GHRP is intended to amplify pulsatile GH release beyond what either produces alone. This acute synergy is pharmacologically reasonable, but the longer-term body-composition, recovery and sleep benefits of this specific blend are not validated by controlled human trials — read such claims as hypotheses (research suggests, may).

Important: Neither CJC-1295 no DAC nor Ipamorelin is an approved medicine. Both are unapproved research chemicals presented here for research and educational purposes only.

06 · Daily habits

Lifestyle Factors

Habits that may support results alongside the protocol.

  • Nutrition: Keep a balanced, protein-rich diet to support muscle and tissue repair.
  • Exercise: Combine resistance and cardiovascular training, scaled to your goals.
  • Sleep: Aim for 7–9 hours; GH pulses are tightly linked to deep sleep.
  • Stress: Manage stress with evidence-based practices to support overall recovery.
07 · What to expect

Potential Benefits & Side Effects

Research suggests possible recovery, body-composition and sleep effects, but human evidence for this blend is limited and individual results vary.

Potential Benefits

  • Recovery & repair: Researchers report improved muscle recovery and repair, attributed to amplified GH pulses.
  • Sleep quality: Anecdotal reports of deeper, more restful sleep.
  • Body composition: May support lean-mass and fat-loss goals over a cycle.
  • Note on humans: These benefits are not validated by controlled trials of this CJC-1295 no DAC + Ipamorelin blend.

Common Side Effects

  • Water retention & flushing: Mild water retention, headaches or transient flushing can occur.
  • Injection-site reactions: Mild redness, tenderness or irritation; rotating sites helps.
  • Unknown long-term profile: Human safety data for this blend is limited, so caution and monitoring are advised.
  • Sport restriction: Both peptides are WADA-prohibited (S2) for athletes.
08 · Injection technique

Injection Technique

General subcutaneous technique, following established clinical best-practice guidance.

Pre-Injection Preparation

  • Wash your hands well with soap and water.
  • Wipe the vial stopper with an alcohol swab and let it air-dry.
  • Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully.
  • Draw the intended total-blend dose, then check for air bubbles and push any out.

Injection Procedure

  • Pinch a skinfold at the chosen site between thumb and forefinger.
  • Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin).
  • Skip aspiration for subcutaneous shots — it isn’t needed.
  • Press the plunger slowly and steadily until it’s fully down.
  • Wait 5–10 seconds, then pull the needle straight out to prevent leakage.

Post-Injection Care

  • Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
  • Return the reconstituted vial to the fridge right away.
  • Rotate the injection site each day to prevent irritation and lipohypertrophy.
  • Watch the site for excess redness, swelling, or signs of infection.
10 · The evidence

References

  1. 1
    Journal of Clinical Endocrinology & Metabolism
    Teichman et al. — CJC-1295 (a GHRH analog) prolongs and elevates GH and IGF-1 in healthy adults.

    View Source

  2. 2
    Endocrine / GHRH pharmacology
    Modified GRF 1-29 (CJC-1295 no DAC) as a short-acting GHRH analog and its pulsatile GH effect.

    View Source

  3. 3
    European Journal of Endocrinology
    Raun et al. — Ipamorelin, a selective ghrelin/GHS-R agonist and GH secretagogue.

    View Source

  4. 4
    GHRH + GHRP synergy
    Studies on combined GHRH and GH-releasing peptide administration amplifying pulsatile GH secretion.

    View Source

  5. 5
    U.S. FDA
    503A bulk drug substances — peptides removed from compounding Category 2 (2024); CJC-1295 and Ipamorelin are not FDA-approved.

    View Source

  6. 6
    World Anti-Doping Agency
    Prohibited List — peptide hormones and GH secretagogues / releasing factors (class S2).

    View Source

  7. 7
    CDC / clinical guidance
    Best practices for safe subcutaneous injection technique and sharps handling.

    View Source

  8. 8
    Peptide handling guidance
    Reconstitution and cold-chain storage practices for lyophilized research peptides.

    View Source

  9. 9
    Prime Lab Peptides
    GROW-H 10 mg blend (CJC-1295 no DAC + Ipamorelin) product page — purity specifications and COAs.

    View Source

Read the complete guide Ipamorelin Dosage: The Complete Protocol Guide (Timing, Reconstitution & Cycle)
Step by step

How to reconstitute CJC-1295 no DAC + Ipamorelin

  1. 1Wipe the vial stopper and your bacteriostatic-water vial with an alcohol swab.
  2. 2Draw your chosen amount of bacteriostatic water and inject it slowly down the inner wall of the CJC-1295 no DAC + Ipamorelin vial.
  3. 3Let it rest, then gently swirl until fully dissolved — do not shake.
  4. 4Refrigerate the reconstituted vial and draw each dose with a U-100 insulin syringe.
FAQ

CJC-1295 no DAC + Ipamorelin — frequently asked questions

How do I reconstitute a 10 mg vial of CJC-1295 no DAC + Ipamorelin?

Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.

How much bacteriostatic water should I add to CJC-1295 no DAC + Ipamorelin?

There is no single correct amount — more water simply spreads the same 10 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.

What do the "units" on an insulin syringe mean?

On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.

How should I store CJC-1295 no DAC + Ipamorelin after mixing?

Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.

How many doses does a 10 mg vial of CJC-1295 no DAC + Ipamorelin provide?

Divide the vial strength of 10 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.

Is CJC-1295 no DAC + Ipamorelin approved for human use?

No. CJC-1295 no DAC + Ipamorelin is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.

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