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AOD-9604 + CJC-1295 + Ipamorelin (12mg Blend Vial) Dosage Protocol

Lipolytic hGH fragment (AOD-9604) + GHRH analog (CJC-1295) + GH secretagogue (Ipamorelin) in one 12 mg blend — research-only, not FDA-approved.

Peptide Stacks Updated November 19, 2025 1 min read Research information only
AOD-9604 + CJC-1295 + Ipamorelin (12mg Blend Vial) Dosage Protocol
Reconstitution calculator

Mix & measure AOD-9604 + CJC-1295 + Ipamorelin · 12 mg

Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers.

Concentrationmg/mL
Draw volumemL
On the syringeunits
Doses / vial 

Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →

Quick answerIn the research literature, the 12 mg AOD-9604 + CJC-1295 + Ipamorelin blend vial is typically reconstituted with 3 mL of bacteriostatic water, and documented research protocols reference roughly 400 mcg per dose drawn from that solution. These figures describe published research handling and are provided strictly for research-use-only purposes.
01 · At a glance

Quickstart Highlights

AOD-9604 + CJC-1295 + Ipamorelin is a single combined 12 mg blend vial pairing AOD-9604 (6 mg; a lipolytic hGH fragment) with CJC-1295 (3 mg; a GHRH analog) and Ipamorelin (3 mg; a selective ghrelin/GHS-R agonist). Together the GH secretagogues are intended to amplify the body’s own pulsatile growth-hormone release while the hGH fragment is studied for fat oxidation. All three are unapproved research chemicals — none is FDA-approved — and the metabolic, regenerative and body-composition benefits of this blend are not validated by controlled human trials. Presented for research and educational use only.

Reconstitute

One 12 mg combined vial. Add 3.0 mL bacteriostatic water → 4 mg/mL (4,000 mcg/mL) total blend.

Daily range

400 mcg → 1.2 mg total blend once daily, titrated upward across a 12-week cycle.

Easy measuring

Doses are total blend. At 4,000 mcg/mL, 1 unit = 40 mcg on a U-100 syringe (units = mcg ÷ 40).

Storage

Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the solution.

Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.

02 · Dosing & reconstitution

Dosing & Reconstitution Guide

One combined 12 mg vial — educational guide for reconstitution and once-daily total-blend dosing

All doses below are stated as total blend (combined AOD-9604 6 mg + CJC-1295 3 mg + Ipamorelin 3 mg). Because all three peptides share one vial, you cannot draw a single component separately. Run once daily with gradual titration across the cycle.

Standard / Gradual Approach (3 mL = 4 mg/mL)

Reconstitute: Add 3.0 mL bacteriostatic water to the 12 mg combined vial4 mg/mL (4,000 mcg/mL).

Typical daily range: 400 mcg (0.4 mg) → 1,200 mcg (1.2 mg) total blend once daily, raised gradually across the cycle.

Easy measuring: At 4,000 mcg/mL, 1 unit = 40 mcg on a U-100 syringe (units = mcg ÷ 40).

Phase / Week(s) Total Blend Dose & Frequency Volume (U-100 units / mL)
Weeks 1–4 400 mcg (0.4 mg) (1× daily) 10 units (0.10 mL)
Weeks 5–8 800 mcg (0.8 mg) (1× daily) 20 units (0.20 mL)
Weeks 9–12 1,200 mcg (1.2 mg) (1× daily) 30 units (0.30 mL)

Frequency: one subcutaneous injection each day at a consistent time, titrating up as tolerated over a 12-week cycle. Measure accurately with a 100-unit insulin syringe; if a calculated dose falls below 10 units, consider a smaller-capacity syringe for improved accuracy. These figures come from common supplier reference protocols, not from approved human dosing.

Reconstitution Steps

1

Draw 3.0 mL of bacteriostatic water into a sterile syringe.

2

Release it slowly down the vial’s inner wall to limit foaming.

3

Swirl or roll gently until fully dissolved — don’t shake.

4

Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.

Note

The 3.0 mL dilution gives a clean 4 mg/mL solution where 1 unit = 40 mcg, keeping the math simple across the titration. Avoid freezing the reconstituted solution, since freeze–thaw can denature the peptides.

Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.

03 · What you’ll need

Supplies Needed

Quantities below assume an 8–16 week course of once-daily injections with gradual titration.

Blend Vials (AOD-9604 + CJC-1295 + Ipamorelin, 12 mg combined)

One combined 12 mg vial. At the titrated once-daily doses, roughly one vial covers a 3–4 week phase at these once-daily doses, so plan several vials for a full course.

  • 8 weeks: ~3 vials
  • 12 weeks: ~6 vials
  • 16 weeks: ~8 vials
Insulin Syringes (U-100, 1 mL)
  • Per injection: 1 syringe
  • 8 weeks: ~56 syringes
  • 12 weeks: ~84 syringes
  • 16 weeks: ~112 syringes
Bacteriostatic Water (30 mL bottle)

Use 3.0 mL per 12 mg vial for reconstitution.

  • Full cycle: 1 × 30 mL bottle
Alcohol Swabs

One for the vial stopper + one for the injection site each day.

  • Per injection: 2 swabs
  • Full cycle: 1 box
AOD-9604 + CJC-1295 + Ipamorelin 12 mg Blend Vial
Blend Vial

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Insulin Syringes
Insulin Syringes

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Bacteriostatic Water
Bacteriostatic Water

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Alcohol Pads
Alcohol Pads

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04 · Good to know

Important Notes

Practical points that keep daily administration safe and consistent.

  • Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a sharps container afterward.
  • One combined vial: CJC-1295 and Ipamorelin share a single vial, so each dose draws both at once — you cannot measure or inject one component separately.
  • Empty stomach: Inject on an empty stomach and wait ~30 minutes before eating; site-rotate across abdomen, thighs and upper arms.
  • Monitoring: Track dose, site and observations weekly; discontinue if serious reactions occur.
  • Regulatory note: Both peptides are research chemicals, not FDA-approved (removed from 503A compounding Category 2 in Sept 2024; status in flux), and both are WADA-prohibited (class S2) for athletes.
05 · How it works

How This Works

AOD-9604 is a fragment of human growth hormone (hGH 176–191) studied for its lipolytic (fat-mobilizing) activity — intended to encourage fat breakdown without the blood-sugar or tissue-growth effects of full-length GH.

CJC-1295 (modified GRF 1-29) is a short-acting GHRH analog. It mimics growth-hormone-releasing hormone at the pituitary for a brief window (~30 minutes), nudging out a pulse of GH without driving a sustained, tonic rise in IGF-1.

Ipamorelin is a selective ghrelin / GHS-R agonist (a growth-hormone-releasing peptide, GHRP). It triggers a clean GH pulse with minimal effect on cortisol or prolactin.

Pairing a GHRH analog with a GHRP is intended to amplify pulsatile GH release beyond what either produces alone. This acute synergy is pharmacologically reasonable, but the longer-term body-composition, recovery and sleep benefits of this specific blend are not validated by controlled human trials — read such claims as hypotheses (research suggests, may).

Important: Neither AOD-9604, CJC-1295 nor Ipamorelin is an approved medicine. Both are unapproved research chemicals presented here for research and educational purposes only.

06 · Daily habits

Lifestyle Factors

Habits that may support results alongside the protocol.

  • Nutrition: Keep a balanced, protein-rich diet to support muscle and tissue repair.
  • Exercise: Combine resistance and cardiovascular training, scaled to your goals.
  • Sleep: Aim for 7–9 hours; GH pulses are tightly linked to deep sleep.
  • Stress: Manage stress with evidence-based practices to support overall recovery.
07 · What to expect

Potential Benefits & Side Effects

Research suggests possible recovery, body-composition and sleep effects, but human evidence for this blend is limited and individual results vary.

Potential Benefits

  • Recovery & repair: Researchers report improved muscle recovery and repair, attributed to amplified GH pulses.
  • Sleep quality: Anecdotal reports of deeper, more restful sleep.
  • Body composition: May support lean-mass and fat-loss goals over a cycle.
  • Note on humans: These benefits are not validated by controlled trials of this CJC-1295 + Ipamorelin blend.

Common Side Effects

  • Water retention & flushing: Mild water retention, headaches or transient flushing can occur.
  • Injection-site reactions: Mild redness, tenderness or irritation; rotating sites helps.
  • Unknown long-term profile: Human safety data for this blend is limited, so caution and monitoring are advised.
  • Sport restriction: Both peptides are WADA-prohibited (S2) for athletes.
08 · Injection technique

Injection Technique

General subcutaneous technique, following established clinical best-practice guidance.

Pre-Injection Preparation

  • Wash your hands well with soap and water.
  • Wipe the vial stopper with an alcohol swab and let it air-dry.
  • Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully.
  • Draw the intended total-blend dose, then check for air bubbles and push any out.

Injection Procedure

  • Pinch a skinfold at the chosen site between thumb and forefinger.
  • Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin).
  • Skip aspiration for subcutaneous shots — it isn’t needed.
  • Press the plunger slowly and steadily until it’s fully down.
  • Wait 5–10 seconds, then pull the needle straight out to prevent leakage.

Post-Injection Care

  • Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
  • Return the reconstituted vial to the fridge right away.
  • Rotate the injection site each day to prevent irritation and lipohypertrophy.
  • Watch the site for excess redness, swelling, or signs of infection.
10 · The evidence

References

  1. 1
    Journal of Clinical Endocrinology & Metabolism
    Teichman et al. — CJC-1295 (a GHRH analog) prolongs and elevates GH and IGF-1 in healthy adults.

    View Source

  2. 2
    Endocrine / GHRH pharmacology
    Modified GRF 1-29 (CJC-1295) as a short-acting GHRH analog and its pulsatile GH effect.

    View Source

  3. 3
    European Journal of Endocrinology
    Raun et al. — Ipamorelin, a selective ghrelin/GHS-R agonist and GH secretagogue.

    View Source

  4. 4
    GHRH + GHRP synergy
    Studies on combined GHRH and GH-releasing peptide administration amplifying pulsatile GH secretion.

    View Source

  5. 5
    U.S. FDA
    503A bulk drug substances — peptides removed from compounding Category 2 (2024); CJC-1295 and Ipamorelin are not FDA-approved.

    View Source

  6. 6
    World Anti-Doping Agency
    Prohibited List — peptide hormones and GH secretagogues / releasing factors (class S2).

    View Source

  7. 7
    CDC / clinical guidance
    Best practices for safe subcutaneous injection technique and sharps handling.

    View Source

  8. 8
    Peptide handling guidance
    Reconstitution and cold-chain storage practices for lyophilized research peptides.

    View Source

  9. 9
    Prime Lab Peptides
    AOD-9604 + CJC-1295 + Ipamorelin blend product line — purity specifications and COAs.

    View Source

Read the complete guide Ipamorelin Dosage: The Complete Protocol Guide (Timing, Reconstitution & Cycle)
Step by step

How to reconstitute AOD-9604 + CJC-1295 + Ipamorelin

  1. 1Wipe the vial stopper and your bacteriostatic-water vial with an alcohol swab.
  2. 2Draw your chosen amount of bacteriostatic water and inject it slowly down the inner wall of the AOD-9604 + CJC-1295 + Ipamorelin vial.
  3. 3Let it rest, then gently swirl until fully dissolved — do not shake.
  4. 4Refrigerate the reconstituted vial and draw each dose with a U-100 insulin syringe.
FAQ

AOD-9604 + CJC-1295 + Ipamorelin — frequently asked questions

How do I reconstitute a 12 mg vial of AOD-9604 + CJC-1295 + Ipamorelin?

Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.

How much bacteriostatic water should I add to AOD-9604 + CJC-1295 + Ipamorelin?

There is no single correct amount — more water simply spreads the same 12 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.

What do the "units" on an insulin syringe mean?

On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.

How should I store AOD-9604 + CJC-1295 + Ipamorelin after mixing?

Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.

How many doses does a 12 mg vial of AOD-9604 + CJC-1295 + Ipamorelin provide?

Divide the vial strength of 12 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.

Is AOD-9604 + CJC-1295 + Ipamorelin approved for human use?

No. AOD-9604 + CJC-1295 + Ipamorelin is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.

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