Ovagen (20mg Vial) Dosage Protocol
Khavinson liver/GI short-peptide bioregulator (EDL) — research-only; not approved, benefits unproven.
Mix & measure Ovagen · 20 mg
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers.
Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →
Quickstart Highlights
Ovagen is a synthetic tripeptide (Glu-Asp-Leu, “EDL”) from the Khavinson family of short-peptide bioregulators, marketed for the liver and digestive system[1][5]. This educational page outlines a once-daily subcutaneous approach with a dilution chosen so doses land on easy-to-read insulin-syringe marks. It is an unapproved research chemical, not a medicine; human evidence is very limited and benefits remain unproven — presented for research and educational use only.
Add 3.0 mL bacteriostatic water to one 20 mg vial → ~6.67 mg/mL (6,667 mcg/mL), a convenient dilution for accurate once-daily dosing.
800–2,000 mcg once daily, titrated upward gradually across an 8–16 week course in commonly cited bioregulator schedules.
At ~6.67 mg/mL, 1 unit ≈ 66.7 mcg; 800 mcg ≈ 12 units and 2,000 mcg ≈ 30 units on a U-100 syringe.
Lyophilized: store at −20 °C (−4 °F); once reconstituted, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the solution.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
Dosing & Reconstitution Guide
A single practical dilution with accurate once-daily dosing, step by step
Standard / Gradual Approach (3 mL = ~6.67 mg/mL)
Reconstitute: Add 3.0 mL bacteriostatic water to one 20 mg vial → final concentration ~6.67 mg/mL (6,667 mcg/mL).
Typical daily range: 800–2,000 mcg once daily, raised gradually over an 8–16 week course.
Easy measuring: At ~6.67 mg/mL, 1 unit ≈ 66.7 mcg on a U-100 syringe. Each dose in this schedule lands between 12 and 30 units on clear syringe markings.
Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the mixed solution.
Frequency: one subcutaneous injection each day, titrating up as tolerated across the 8–16 week course, in line with commonly cited bioregulator schedules[3][4]. These figures come from vendor and reference protocols, not from approved human dosing or independent clinical trials.
Reconstitution Steps
Draw 3.0 mL of bacteriostatic water into a sterile syringe.
Release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.
The 3.0 mL dilution keeps each dose on clear U-100 syringe markings (about 12–30 units). Avoid freezing the reconstituted solution, since freeze–thaw can denature the peptide.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
Quantities below assume an 8–16 week course of once-daily injections with gradual titration.
A 20 mg vial covers roughly two weeks of dosing at the doses shown, so plan about one vial every two weeks.
- 8 weeks: ~4 vials
- 12 weeks: ~6 vials
- 16 weeks: ~8 vials
- Per injection: 1 syringe
- 8 weeks (once daily): ~56 syringes
- 16 weeks (once daily): ~112 syringes
Use ~3.0 mL per 20 mg vial for reconstitution.
- 8 weeks (4 vials): ~12 mL → 2 bottles
- 16 weeks (8 vials): ~24 mL → 3 bottles
One for the vial stopper + one for the injection site each day.
- Per injection: 2 swabs
- 8 weeks (once daily): ~112 swabs → 1–2 boxes
Protocol Overview
A concise summary of the once-daily regimen, drawn from commonly cited bioregulator reference protocols.
- ▪Goal: Marketed as a liver/GI short-peptide bioregulator under a proposed peptide-DNA gene-regulation hypothesis — this mechanism is the originators’ hypothesis, not independently confirmed, and benefits are not established in humans[5][6].
- ▪Schedule: Daily subcutaneous injections for 8–16 weeks per commonly cited bioregulator courses.
- ▪Dose Range: 800–2,000 mcg per day with gradual titration across the course.
- ▪Reconstitution: 3.0 mL bacteriostatic water per 20 mg vial gives ~6.67 mg/mL for accurate unit measurements.
- ▪Storage: Keep the dry vial frozen at −20 °C (−4 °F); once mixed, refrigerate at 2–8 °C and do not freeze the solution.
Dosing Protocol
A suggested daily titration approach based on common reference doses.
- ▪Start: Begin at 800 mcg once daily to gauge tolerability.
- ▪Titrate: Increase by roughly 400 mcg every two weeks as tolerated.
- ▪Target: Reach about 2,000 mcg daily by weeks 7–12.
- ▪Cycle Length: Typically 8–16 weeks per cited bioregulator courses.
- ▪Timing: Inject at a consistent time each day and rotate injection sites systematically.
Storage Instructions
Correct storage is what preserves the peptide’s stability and activity.
- ▪Lyophilized: Hold the dry vial at −20 °C (−4 °F) in dry, dark conditions and limit moisture exposure[7].
- ▪Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F) and use within about 28 days; do not freeze the mixed solution, as freezing can denature peptides[8].
- ▪Handling: Let frozen vials warm to room temperature before opening so condensation won’t form, and keep the solution clear of heat and direct light.
- ▪Freeze–thaw: Avoid repeated freeze–thaw cycles of the reconstituted solution.
Important Notes
Practical points that keep daily administration safe and consistent.
- ▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward.
- ▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation and lipohypertrophy[9].
- ▪Slow injection: Push the plunger slowly and pause a few seconds before withdrawing the needle to prevent backflow.
- ▪Recordkeeping: Log the daily dose, injection site and any observations to keep the protocol consistent.
- ▪Regulatory note: Ovagen (EDL) is not FDA- or EMA-approved for human use, and as an unapproved substance it falls under WADA’s catch-all (S0, possibly S2) for competitive sport[10].
How This Works
Ovagen is a synthetic tripeptide with the sequence Glu-Asp-Leu (EDL)[1][2]. It belongs to the Khavinson family of short-peptide bioregulators and is marketed for the liver, gastrointestinal tract and digestive system.
Its proposed mechanism is peptide-DNA gene regulation: the originators hypothesize that short peptides such as EDL bind regulatory regions of DNA and influence tissue-specific gene expression in liver and gut cells[5][6]. This is a hypothesis advanced by the originating group, not an independently confirmed mechanism.
The reported liver and digestive-system effects come almost entirely from a single Russian research group plus vendor materials[11], with no independent randomized controlled trials and very limited, low-quality human data[12].
Important caveat: there are no independent human efficacy trials of Ovagen (EDL). “Hepatocyte resilience”, “organ rejuvenation” and anti-aging claims should be read as unproven hypotheses, not established effects. Note on the name: a separate, unrelated product is also called “Ovagen” — an ovine-FSH preparation used to induce ovulation/superovulation in animals. This page is the EDL tripeptide bioregulator, not the FSH product, and the animal-ovulation literature does not apply here.
Ovagen is not an approved medicine. It is an unapproved research chemical presented here for research and educational purposes only.
Lifestyle Factors
General wellbeing habits that may support overall health alongside the protocol.
- ▪Nutrition: Favor a balanced diet that supports liver and digestive health, limiting alcohol and excess processed food.
- ▪Activity & rest: Pair regular movement with adequate rest as part of a sustainable routine.
- ▪Sleep: Aim for 7–9 hours to support the body’s natural maintenance processes.
- ▪Stress: Manage stress with evidence-based practices, since it influences overall health.
Potential Benefits & Side Effects
What vendor and originator literature describe; independent human evidence is very limited and results are unproven.
Potential Benefits
- ▪Liver/GI claims (unproven): Originator and vendor sources describe support for hepatocyte function and digestive health, framed through the peptide-DNA hypothesis — not independently confirmed[5][6].
- ▪Evidence base (weak): Reported effects rest almost entirely on a single research group plus vendor content, with no independent RCTs[11].
- ▪Tolerability (limited data): Short-peptide bioregulators are generally described as well tolerated, with occasional mild injection-site reactions, but safety data are sparse.
- ▪Note on humans: These benefits are not established in humans — no independent randomized controlled trials of Ovagen (EDL) have been completed[13].
Common Side Effects
- ▪Injection-site reactions: Mild redness, tenderness or soreness can occur; rotating sites helps.
- ▪Unknown long-term profile: Human safety data is limited, so caution and monitoring are advised.
- ▪Sport restriction: As an unapproved substance, Ovagen falls under WADA’s catch-all prohibition (S0) for athletes.
Injection Technique
General subcutaneous technique, following established clinical best-practice guidance[14][15].
Pre-Injection Preparation
- ▪Wash your hands well with soap and water.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[15].
- ▪Draw the intended dose, then check for air bubbles and push any out.
Injection Procedure
- ▪Pinch a skinfold at the chosen site between thumb and forefinger.
- ▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[14].
- ▪Skip aspiration for subcutaneous shots — it isn’t needed[14].
- ▪Press the plunger slowly and steadily until it’s fully down.
- ▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
Post-Injection Care
- ▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away.
- ▪Rotate the injection site each day to prevent irritation and lipohypertrophy[9].
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
For high-purity research peptides and bacteriostatic water, we point researchers to Prime Lab Peptides.
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
Khavinson short peptides (PubMed)Overview of Khavinson short-peptide bioregulators (including EDL) and the proposed peptide-DNA gene-regulation concept.
- 2
EDL peptide (PubMed)Studies on the Glu-Asp-Leu (EDL) tripeptide reported for liver/hepatoprotective activity by the originating group.
- 3
Peptide bioregulators review (PubMed)Review of peptide bioregulators and proposed dosing/course conventions in the originator literature.
- 4
Bioregulator dosing conventions (PubMed)Commonly cited short-peptide course lengths and dosing conventions; figures derive from vendor/originator protocols, not approved dosing.
- 5
Peptide-DNA interaction hypothesis (PubMed)Originator papers proposing that short peptides interact with DNA to regulate tissue-specific gene expression — a hypothesis, not independently confirmed.
- 6
Short-peptide gene-regulation review (PubMed)Reviews of proposed short-peptide gene-regulatory mechanisms; evidence is preliminary and largely from one group.
- 7
Peptide Storage GuideBest practices for storing lyophilized peptides (temperature, humidity and light protection).
- 8
Bacteriostatic Water GuidanceBacteriostatic water for injection: multi-dose vial stability and handling.
- 9
NCBI BookshelfBest practices for subcutaneous injection: aseptic technique and site rotation.
- 10
WADA Prohibited ListWADA prohibited list — unapproved substances fall under the S0 catch-all (and possibly S2) for competitive sport.
- 11
Single-group / vendor evidence (PubMed)Liver/GI claims for EDL trace largely to one Russian research group and vendor materials; no independent replication.
- 12
Evidence-quality appraisal (PubMed)Critical appraisal of peptide-bioregulator literature noting small studies, methodological limits and lack of independent RCTs.
- 13
ClinicalTrials.govTrial registry search; no completed independent efficacy trials of the Ovagen (EDL) tripeptide are listed.
- 14
Centers for Disease Control and Prevention (CDC)Subcutaneous injection technique: angle, site and no-aspiration guidance.
- 15
Subcutaneous Injection Technique (Patient Education)How to administer a subcutaneous injection: clinical technique guidelines.
- 16
Prime Lab PeptidesResearch-peptide supplier — purity specifications and certificates of analysis.
How to reconstitute Ovagen
- 1Wipe the vial stopper and your bacteriostatic-water vial with an alcohol swab.
- 2Draw your chosen amount of bacteriostatic water and inject it slowly down the inner wall of the Ovagen vial.
- 3Let it rest, then gently swirl until fully dissolved — do not shake.
- 4Refrigerate the reconstituted vial and draw each dose with a U-100 insulin syringe.
Ovagen — frequently asked questions
How do I reconstitute a 20 mg vial of Ovagen?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to Ovagen?
There is no single correct amount — more water simply spreads the same 20 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store Ovagen after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 20 mg vial of Ovagen provide?
Divide the vial strength of 20 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is Ovagen approved for human use?
No. Ovagen is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
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